Evidence map›Paper›PMID 28018584›Full record

ArticleBoneKEy reports2016

Confocal/two-photon microscopy in studying colonisation of cancer cells in bone using xenograft mouse models.

Gloria Allocca, Anjali P Kusumbe, Saravana K Ramasamy, Ning Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BoneKEy reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Deep imaging of LepRBone research · 2025
    Article
  2. Review
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Gloria AlloccaDepartment of Oncology and Metabolism, The Mellanby Centre for Bone Research, The University of Sheffield , Sheffield, UK.
Anjali P KusumbeKennedy Institute of Rheumatology, University of Oxford , Oxford, UK.
Saravana K RamasamyIntegrative Skeletal Physiology group, Institute of Clinical Sciences, Imperial College London, London, UK; MRC Clinical Sciences Centre, Du Cane Road, London, UK.
Ning WangDepartment of Oncology and Metabolism, The Mellanby Centre for Bone Research, The University of Sheffield , Sheffield, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Confocal and two-photon microscopy has been widely used in bone research to not only produce high quality, three-dimensional images but also to provide valuable structural and quantitative information. In this article, we describe step-by-step protocols for confocal and two-photon microscopy to investigate earlier cellular events during colonisation of cancer cells in bone using xenograft mouse models. This includes confocal/two-photon microscopy imaging of paraformaldehyde fixed thick bone sections and frozen bone samples.

Identifiers

PMID28018584
PMCPMC5141599

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.