ArticleJournal of biological engineering2016
Can terminators be used as insulators into yeast synthetic gene circuits?
Article in Journal of biological engineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- K48-ubiquitin-dependent proteases cut-up post-ER proteins.Nature communications · 2026Article
- Efficient sex hormone biosensors in Saccharomyces cerevisiae cells to evaluate human aromatase activity and inhibition.Scientific reports · 2025Article
- Hybrid Synthetic Promoters in Saccharomyces cerevisiae Built on Foreign Promoter Sequences.Methods in molecular biology (Clifton, N.J.) · 2024Article
- RNAi-based Boolean gates in the yeastFrontiers in bioengineering and biotechnology · 2024Article
- Refactoring the architecture of a polyketide gene cluster enhances docosahexaenoic acid production in Yarrowia lipolytica through improved expression and genetic stability.Microbial cell factories · 2023Article
- Multidimensional characterization of inducible promoters and a highly light-sensitive LOV-transcription factor.Nature communications · 2023Article
- Development of Terminator-Promoter Bifunctional Elements for Application inInternational journal of molecular sciences · 2023Article
- Hybrid Boolean gates show that Cas12c controls transcription activation effectively in the yeastFrontiers in bioengineering and biotechnology · 2023Article
- Engineering a two-gene system to operate as a highly sensitive biosensor or a sharp switch upon induction with β-estradiol.Scientific reports · 2022Article
- Article
- Tuning heterologous glucan biosynthesis in yeast to understand and exploit plant starch diversity.BMC biology · 2022Article
- A Mutated Nme1Cas9 Is a Functional Alternative RNase to Both LwaCas13a and RfxCas13d in the YeastFrontiers in bioengineering and biotechnology · 2022Article
- NOT Gates Based on Protein Degradation as a Case Study for a New Modular Modeling via SBML Level 3-Comp Package.Frontiers in bioengineering and biotechnology · 2022Article
- Review
- NovelInternational journal of molecular sciences · 2021Article
- Genetic circuit design automation for yeast.Nature microbiology · 2020Article
- Systems and Synthetic Biology Approaches to Engineer Fungi for Fine Chemical Production.Frontiers in bioengineering and biotechnology · 2018Review
- Anti-CRISPR-based biosensors in the yeastJournal of biological engineering · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn bacteria, transcription units can be insulated by placing a terminator in front of a promoter. In this way promoter leakage due to the read-through from an upstream gene or RNA polymerase unspecific binding to the DNA is, in principle, removed. Differently from bacterial terminators, yeast
resultsBy placing different yeast terminators (natural and synthetic) in front of the CYC1 yeast constitutive promoter stripped of every upstream activating sequences and TATA boxes, we verified that the efficiency element is able to bind RNA polymerase II, hence working as a TATA box. Moreover, terminators put in front of strong and medium-strength constitutive yeast promoters cause a non-negligible decrease in the promoter transcriptional activity.
conclusionsOur data suggests that RNA polymerase II molecules upon binding the insulator efficiency element interfere with protein expression by competing either with activator proteins at the promoter enhancers or other RNA polymerase II molecules targeting the TATA box. Hence, it seems preferable to avoid the insulation of non-weak promoters when building synthetic gene circuit in yeast
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.