ArticleStem cell reports2017
Direct Reprogramming of Mouse Fibroblasts toward Leydig-like Cells by Defined Factors.
Article in Stem cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 52 citations in OpenAlex.
- Dynamic Landscape of H3K4me3 and H3K27me3 Modification During Postnatal Leydig Cell Fate Determination.Andrology · 2026Article
- Functional and transcriptomic insights into 46,XY disorders of sex development associated with NR5A1 gene variants.Biology of sex differences · 2026Article
- High resolution tissue and cell type identification via single cell transcriptomic profiling.PloS one · 2025Article
- Generation of glucocorticoid-producing cells derived from human pluripotent stem cells.Cell reports methods · 2023Article
- Small Molecule Cocktails Promote Fibroblast-to-Leydig-like Cell Conversion for Hypogonadism Therapy.Pharmaceutics · 2023Article
- Strategies in Infertile Azoospermic Patients with Negative Microdissection Testicular Sperm Extraction Surgery.Urology research & practice · 2023Article
- High-fidelity reprogramming into Leydig-like cells by CRISPR activation and paracrine factors.PNAS nexus · 2022Article
- Emerging concepts on Leydig cell development in fetal and adult testis.Frontiers in endocrinology · 2022Review
- Transcription Factors in the Regulation of Leydig Cell Gene Expression and Function.Frontiers in endocrinology · 2022Review
- Advances in stem cell research for the treatment of primary hypogonadism.Nature reviews. Urology · 2021Review
- An In Vitro Differentiation Protocol for Human Embryonic Bipotential Gonad and Testis Cell Development.Stem cell reports · 2020Article
- Coordination of Multiple Cellular Processes by NR5A1/Nr5a1.Endocrinology and metabolism (Seoul, Korea) · 2020Review
- Stem cell-based therapies for fertility preservation in males: Current status and future prospects.World journal of stem cells · 2020Review
- Conversion of Fibroblast into Functional Leydig-like Cell Using Defined Small Molecules.Stem cell reports · 2020Article
- Characterization of rodent Sertoli cell primary cultures.Molecular reproduction and development · 2020Review
- Leydig-like cells derived from reprogrammed human foreskin fibroblasts by CRISPR/dCas9 increase the level of serum testosterone in castrated male rats.Journal of cellular and molecular medicine · 2020Article
- Reprogramming of human fibroblasts into osteoblasts by insulin-like growth factor-binding protein 7.Stem cells translational medicine · 2020Article
- Stem Leydig Cells in the Adult Testis: Characterization, Regulation and Potential Applications.Endocrine reviews · 2020Review
- Direct cell-fate conversion of somatic cells: Toward regenerative medicine and industries.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2020Review
- CRISPR/dCas9-mediated activation of multiple endogenous target genes directly converts human foreskin fibroblasts into Leydig-like cells.Journal of cellular and molecular medicine · 2019Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leydig cells (LCs) play crucial roles in producing testosterone, and their dysfunction leads to male hypogonadism. LC transplantation is a promising alternative therapy for male hypogonadism. However, the source of LCs limits this strategy for clinical applications. Here, we report our success in reprogramming mice fibroblasts into LCs by expressing three transcriptional factors, Dmrt1, Gata4, and Nr5a1. The induced Leydig-like cells (iLCs) expressed steroidogenic genes, had a global gene expression profile similar to that of adult LCs, and acquired androgen synthesis capabilities. When iLCs were transplanted into rats or mice testes that were selectively depleted of endogenous LCs, the transplanted cells could survive and function in the interstitium of testis, resulting in the restoration of normal levels of serum testosterone. These findings demonstrate that the fibroblasts were able to be directly converted into iLCs by few defined factors, which may facilitate future applications in regenerative medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.