Evidence map›Paper›PMID 28013394›Full record

ArticleJournal of industrial microbiology & biotechnology2017

Purification, characterization, and application of a thermostable dextranase from Talaromyces pinophilus.

Yu-Qi Zhang, Ruo-Han Li, Hong-Bin Zhang, Min Wu, Xue-Qin Hu

Abstract read
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In one paragraph

Article in Journal of industrial microbiology & biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
5.1field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 39 citations in OpenAlex.

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  7. The MarineMarine drugs · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Yu-Qi ZhangDepartment of Pharmaceutical Engineering, School of Biological and Medical Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China.
Ruo-Han LiDepartment of Pharmaceutical Engineering, School of Biological and Medical Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China.
Hong-Bin ZhangDepartment of Pharmaceutical Engineering, School of Biological and Medical Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. hbzhang@hfut.edu.cn.
Min WuDepartment of Pharmaceutical Engineering, School of Biological and Medical Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China.
Xue-Qin HuDepartment of Pharmaceutical Engineering, School of Biological and Medical Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China.
Hefei University of Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Dextranase can hydrolyze dextran to low-molecular-weight polysaccharides, which have important medical applications. In the study, dextranase-producing strains were screened from various soil sources. The strain H6 was identified as Talaromyces pinophilus by a standard ITS rDNA analysis. Crude dextranase was purified by ammonium sulfate fractionation and Sepharose 6B chromatography, which resulted in a 6.69-fold increase in the specific activity and an 11.27% recovery. The enzyme was 58 kDa, lower than most dextranase, with an optimum temperature of 45 °C and an optimum pH of 6.0, and identified as an endodextranase. It was steady over a pH range from 3.0 to 10.0 and had reasonable thermal stability. The dextranase activity was increased by urea, which enhanced its activity to 115.35% and was conducive to clinical dextran production. Therefore, T. pinophilus H6 dextranase could show its superiority in practical applications.

Indexed as

Culture MediaDextranaseDextransGlucansHydrogen-Ion ConcentrationHydrolysisIndustrial MicrobiologyMolecular WeightSoil MicrobiologyTalaromycesCulture MediaDextranaseDextransGlucansCharacterizationDextranasePurificationTalaromyces pinophilusα-Glucan degradation

Identifiers

PMID28013394
OpenAlexW2562927311

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.