ArticleMolecular biotechnology2017
A Synthetic Hybrid Promoter for Xylose-Regulated Control of Gene Expression in Saccharomyces Yeasts.
Article in Molecular biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
- Development of a xylose-inducible and glucose-insensitive expression system for Parageobacillus thermoglucosidasius.Applied microbiology and biotechnology · 2024Article
- Efficient Production of Glucaric Acid by Engineered Saccharomyces cerevisiae.Applied and environmental microbiology · 2023Article
- D-xylose accelerated death of pentose metabolizing Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2023Article
- Review
- Regulation of Transgene Expression by the Natural Sweetener Xylose.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Development of a Glycerol-Inducible Expression System for High-Yield Heterologous Protein Production in Bacillus subtilis.Microbiology spectrum · 2022Article
- Engineering of Synthetic Transcriptional Switches in Yeast.Life (Basel, Switzerland) · 2022Review
- Valorisation of xylose to renewable fuels and chemicals, an essential step in augmenting the commercial viability of lignocellulosic biorefineries.Sustainable energy & fuels · 2021Review
- D-Xylose Sensing inInternational journal of molecular sciences · 2021Review
- Review
- Engineering metabolite-responsive transcriptional factors to sense small molecules in eukaryotes: current state and perspectives.Microbial cell factories · 2019Review
- In vivo biosensors: mechanisms, development, and applications.Journal of industrial microbiology & biotechnology · 2018Article
- Condition-specific promoter activities in Saccharomyces cerevisiae.Microbial cell factories · 2018Article
- Systems and Synthetic Biology Approaches to Engineer Fungi for Fine Chemical Production.Frontiers in bioengineering and biotechnology · 2018Review
- Lighting up yeast cell factories by transcription factor-based biosensors.FEMS yeast research · 2017Article
- Influence of genetic background of engineered xylose-fermenting industrial Saccharomyces cerevisiae strains for ethanol production from lignocellulosic hydrolysates.Journal of industrial microbiology & biotechnology · 2017Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolism of non-glucose carbon sources is often highly regulated at the transcriptional and post-translational levels. This level of regulation is lacking in Saccharomyces cerevisiae strains engineered to metabolize xylose. To better control transcription in S. cerevisiae, the xylose-dependent, DNA-binding repressor (XylR) from Caulobacter crescentus was used to block transcription from synthetic promoters based on the constitutive Ashbya gossypii TEF promoter. The new hybrid promoters were repressed in the absence of xylose and showed up to a 25-fold increase in the presence of xylose. Activation of the promoter was highly sensitive to xylose with activity seen at concentrations below 2 μM xylose. These new xylose-inducible promoters allow improved control of gene expression for engineered strains of Saccharomyces yeasts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.