ArticleMicrobial cell factories2016
Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 89 citations in OpenAlex.
- Metabolic engineering strategies: utilizing different microbial strains and advanced technologies for the synthesis of high-valued terpenoids.Antonie van Leeuwenhoek · 2026Review
- Increased Mevalonate Production Using Engineered Citrate Synthase and Phosphofructokinase Variants of Escherichia coli.Biotechnology and bioengineering · 2025Article
- Stable Platform for Mevalonate Bioproduction from COACS sustainable chemistry & engineering · 2024Article
- Comparative whole genome analysis of face-derived Streptococcus infantis CX-4 unravels the functions related to skin barrier.Genes & genomics · 2024Article
- Rational engineering ofFrontiers in microbiology · 2024Article
- Advances in the Discovery and Engineering of Gene Targets for Carotenoid Biosynthesis in Recombinant Strains.Biomolecules · 2023Review
- Optimization of IspSApplied and environmental microbiology · 2023Article
- Engineered production of isoprene from the model green microalgaMetabolic engineering communications · 2023Article
- Article
- Carotenoids in Drug Discovery and Medicine: Pathways and Molecular Targets Implicated in Human Diseases.Molecules (Basel, Switzerland) · 2022Review
- MICROBIAL isoprene production: an overview.World journal of microbiology & biotechnology · 2022Review
- Microbial Production Potential ofMicroorganisms · 2022Review
- Non-enzymatic formation of isoprene and 2-methyl-3-buten-2-ol (2-MBO) by manganese.Scientific reports · 2022Article
- Rationally optimized generation of integratedSynthetic and systems biotechnology · 2021Article
- New aspects of microbial vitamin K2 production by expanding the product spectrum.Microbial cell factories · 2021Review
- Evolution of isoprene emission in Arecaceae (palms).Evolutionary applications · 2021Article
- Impact of culture condition modulation on the high-yield, high-specificity and cost-effective production of terpenoids from microbial sources: A review.Applied and environmental microbiology · 2021Review
- Anaerobic Production of Isoprene by EngineeredApplied and environmental microbiology · 2021Article
- Enriching the Production of 2-Methyl-1-Butanol in Fermentation Process Using Corynebacterium crenatum.Current microbiology · 2020Article
- Modular engineering for microbial production of carotenoids.Metabolic engineering communications · 2020Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIsoprene, a volatile C5 hydrocarbon, is an important platform chemical used in the manufacturing of synthetic rubber for tires and various other applications, such as elastomers and adhesives.
resultsIn this study, Escherichia coli MG1655 harboring Populus trichocarpa isoprene synthase (PtispS) and the exogenous mevalonate (MVA) pathway produced 80 mg/L isoprene. Codon optimization and optimal expression of the ispS gene via adjustment of the RBS strength and inducer concentration increased isoprene production to 199 and 337 mg/L, respectively. To augment expression of MVA pathway genes, the MVA pathway was cloned on a high-copy plasmid (pBR322 origin) with a strong promoter (P
conclusionsWe achieved a 23-fold increase in isoprene production by codon optimization of PtispS, augmentation of the MVA pathway, and deletion of genes involved in byproduct formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.