ArticleJournal of synchrotron radiation2017
Development of a dose-limiting data collection strategy for serial synchrotron rotation crystallography.
Article in Journal of synchrotron radiation, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Macromolecular crystallography at SPring-8 and SACLA.Journal of synchrotron radiation · 2025Article
- Useful experimental aspects of small-wedge synchrotron crystallography for accurate structure analysis of protein molecules.Acta crystallographica. Section D, Structural biology · 2025Article
- Review of serial femtosecond crystallography including the COVID-19 pandemic impact and future outlook.Structure (London, England : 1993) · 2023Review
- Elucidating polymorphs of crystal structures by intensity-based hierarchical clustering analysis of multiple diffraction data sets.Acta crystallographica. Section D, Structural biology · 2023Article
- How Beam Damage Can Skew SynchrotronACS energy letters · 2023Article
- Cell-free protein crystallization for nanocrystal structure determination.Scientific reports · 2022Article
- Serial crystallography with multi-stage merging of thousands of images.Acta crystallographica. Section F, Structural biology communications · 2022Article
- Single-support serial isomorphous replacement phasing.Acta crystallographica. Section D, Structural biology · 2022Article
- In situ crystal data-collection and ligand-screening system at SPring-8.Acta crystallographica. Section F, Structural biology communications · 2022Article
- Quantifying and comparing radiation damage in the Protein Data Bank.Nature communications · 2022Article
- Implementation of wedged-serial protein crystallography at PROXIMA-1.Journal of synchrotron radiation · 2022Article
- 3D Electron Diffraction for Chemical Analysis: Instrumentation Developments and Innovative Applications.Chemical reviews · 2021Review
- Guidelines for de novo phasing using multiple small-wedge data collection.Journal of synchrotron radiation · 2021Article
- Integrated sample-handling and mounting system for fixed-target serial synchrotron crystallography.Acta crystallographica. Section D, Structural biology · 2021Article
- Evaluation of the data-collection strategy for room-temperature micro-crystallography studied by serial synchrotron rotation crystallography combined with the humid air and glue-coating method.Acta crystallographica. Section D, Structural biology · 2021Article
- Isoprenoid-chained lipid EROCOCScientific reports · 2020Article
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- The domain swapping of human cystatin C induced by synchrotron radiation.Scientific reports · 2019Article
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10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Serial crystallography, in which single-shot diffraction images are collected, has great potential for protein microcrystallography. Although serial femtosecond crystallography (SFX) has been successfully demonstrated, limited beam time prevents its routine use. Inspired by SFX, serial synchrotron crystallography (SSX) has been investigated at synchrotron macromolecular crystallography beamlines. Unlike SFX, the longer exposure time of milliseconds to seconds commonly used in SSX causes radiation damage. However, in SSX, crystals can be rotated during the exposure, which can achieve efficient coverage of the reciprocal space. In this study, mercury single-wavelength anomalous diffraction (Hg-SAD) phasing of the luciferin regenerating enzyme (LRE) was performed using serial synchrotron rotation crystallography. The advantages of rotation and influence of dose on the data collected were evaluated. The results showed that sample rotation was effective for accurate data collection, and the optimum helical rotation step depended on multiple factors such as multiplicity and partiality of reflections, exposure time per rotation angle and the contribution from background scattering. For the LRE microcrystals, 0.25° was the best rotation step for the achievable resolution limit, whereas a rotation step larger than or equal to 1° was favorable for Hg-SAD phasing. Although an accumulated dose beyond 1.1 MGy caused specific damage at the Hg site, increases in resolution and anomalous signal were observed up to 3.4 MGy because of a higher signal-to-noise ratio.
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