ArticleStem cells (Dayton, Ohio)2017
New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells.
Article in Stem cells (Dayton, Ohio), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
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- The distribution of neuroligin4, an autism-related postsynaptic molecule, in the human brain.Molecular brain · 2023Article
- Benchmarking of ATAC Sequencing Data From BGI's Low-Cost DNBSEQ-G400 Instrument for Identification of Open and Occupied Chromatin Regions.Frontiers in molecular biosciences · 2022Article
- Desmoglein 2 regulates cardiogenesis by restricting hematopoiesis in the developing murine heart.Scientific reports · 2021Article
- Article
- Development of a High-Efficacy Reprogramming Method for Generating Human Induced Pluripotent Stem (iPS) Cells from Pathologic and Senescent Somatic Cells.International journal of molecular sciences · 2020Article
- Photoinduced reconfiguration to control the protein-binding affinity of azobenzene-cyclized peptides.Journal of materials chemistry. B · 2020Article
- Cell-Surface Proteomics Identifies Differences in Signaling and Adhesion Protein Expression between Naive and Primed Human Pluripotent Stem Cells.Stem cell reports · 2020Article
- The application of cell surface markers to demarcate distinct human pluripotent states.Experimental cell research · 2020Review
- Single-Cell Profiling Identifies Key Pathways Expressed by iPSCs Cultured in Different Commercial Media.iScience · 2018Article
- H1N1 influenza virus epitopes classified by monoclonal antibodies.Experimental and therapeutic medicine · 2018Article
- Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming.Nature methods · 2017Article
Corrections and comments
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Authors and funding
27 authors at 11 institutions in 4 countries.
Funding
Abstract
The study and application of human pluripotent stem cells (hPSCs) will be enhanced by the availability of well-characterized monoclonal antibodies (mAbs) detecting cell-surface epitopes. Here, we report generation of seven new mAbs that detect cell surface proteins present on live and fixed human ES cells (hESCs) and human iPS cells (hiPSCs), confirming our previous prediction that these proteins were present on the cell surface of hPSCs. The mAbs all show a high correlation with POU5F1 (OCT4) expression and other hPSC surface markers (TRA-160 and SSEA-4) in hPSC cultures and detect rare OCT4 positive cells in differentiated cell cultures. These mAbs are immunoreactive to cell surface protein epitopes on both primed and naive state hPSCs, providing useful research tools to investigate the cellular mechanisms underlying human pluripotency and states of cellular reprogramming. In addition, we report that subsets of the seven new mAbs are also immunoreactive to human bone marrow-derived mesenchymal stem cells (MSCs), normal human breast subsets and both normal and tumorigenic colorectal cell populations. The mAbs reported here should accelerate the investigation of the nature of pluripotency, and enable development of robust cell separation and tracing technologies to enrich or deplete for hPSCs and other human stem and somatic cell types. Stem Cells 2017;35:626-640.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.