ArticleScientific reports2016
Biochemical and structural characterization of a novel halotolerant cellulase from soil metagenome.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 83 citations in OpenAlex.
- Recombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications.Microbial cell factories · 2021Pooled it
- Unlocking Enzyme Discovery: Leveraging Multi-Omics, Machine Learning, and De Novo Design.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Genome-Wide Identification and In Silico Analysis of Annexins in Chickpea (Cicer arietinum L.).Biochemical genetics · 2025Article
- Characterization of a novel halophilic and thermostable multifunctional cellulase from Ebinur Salt Lake.Scientific reports · 2025Article
- Genomic Functional Analysis and Cellulase Characterization for the Enzyme-Producing StrainMicroorganisms · 2025Article
- How sensitive are protein hydration shells to electrolyte concentration and protein composition?Protein science : a publication of the Protein Society · 2025Article
- Precision enzyme discovery through targeted mining of metagenomic data.Natural products and bioprospecting · 2024Review
- High NaCl concentrations induce the resistance to thermal denaturation of an extremely halotolerant (salt-activated) β-mannanase from Bacillus velezensis H1.World journal of microbiology & biotechnology · 2023Article
- Biochemical and structural characterisation of a family GH5 cellulase from endosymbiont of shipworm P. megotara.Biotechnology for biofuels and bioproducts · 2023Article
- Production of a halotolerant endo-1,4-β-glucanase by a newly isolated Bacillus velezensis H1 on olive mill wastes without pretreatment: purification and characterization of the enzyme.Archives of microbiology · 2022Article
- Characterization of in vitro stability for two processive endoglucanases as exogenous fibre biocatalysts in pig nutrition.Scientific reports · 2022Article
- Discovery of novel carbohydrate degrading enzymes from soda lakes through functional metagenomics.Frontiers in microbiology · 2022Article
- In silico characterization of the GH5-cellulase family from uncultured microorganisms: physicochemical and structural studies.Journal, genetic engineering & biotechnology · 2021Article
- Identification and characterization of a novel carboxylesterase EstQ7 from a soil metagenomic library.Archives of microbiology · 2021Article
- Current Status of Mining, Modification, and Application of Cellulases in Bioactive Substance Extraction.Current issues in molecular biology · 2021Review
- Molecular cloning and characterization of a thermostable and halotolerant endo-β-1,4-glucanase from3 Biotech · 2021Article
- Efficient biomass saccharification using a novel cellobiohydrolase fromRSC advances · 2021Article
- A novel high performance in-silico screened metagenome-derived alkali-thermostable endo-β-1,4-glucanase for lignocellulosic biomass hydrolysis in the harsh conditions.BMC biotechnology · 2020Article
- Understanding High-Salt and Cold Adaptation of a Polyextremophilic Enzyme.Microorganisms · 2020Article
- Engineering of a highly thermostable endoglucanase from the GH7 family of Bipolaris sorokiniana for higher catalytic efficiency.Applied microbiology and biotechnology · 2020Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellulase catalyzes the hydrolysis of β-1,4-linkages of cellulose to produce industrially relevant monomeric subunits. Cellulases find their applications in pulp and paper, laundry, food and feed, textile, brewing industry and in biofuel production. These industries always have great demand for cellulases that can work efficiently even in harsh conditions such as high salt, heat, and acidic environments. While, cellulases with high thermal and acidic stability are already in use, existence of a high halotolerant cellulase is still elusive. Here, we report a novel cellulase Cel5R, obtained from soil metagenome that shows high halotolerance and thermal stability. The biochemical and functional characterization of Cel5R revealed its endoglucanase activity and high halostability. In addition, the crystal structure of Cel5R determined at 2.2 Å resolution reveals a large number of acidic residues on the surface of the protein that contribute to the halophilic nature of this enzyme. Moreover, we demonstrate that the four free and non-conserved cysteine residues (C65, C90, C231 and C273) contributes to the thermal stability of Cel5R by alanine scanning experiments. Thus, the newly identified endoglucanase Cel5R is a promising candidate for various industrial applications.
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