ArticleEMBO reports2017
Changing POU dimerization preferences converts Oct6 into a pluripotency inducer.
Article in EMBO reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 47 citations in OpenAlex.
- The emergence of Sox and POU transcription factors predates the origins of animal stem cells.Nature communications · 2024Article
- Oct4 redox sensitivity potentiates reprogramming and differentiation.Genes & development · 2024Article
- Mechanisms, pathways and strategies for rejuvenation through epigenetic reprogramming.Nature aging · 2024Review
- The homeodomain of Oct4 is a dimeric binder of methylated CpG elements.Nucleic acids research · 2023Article
- Article
- SOX9 and SOX10 control fluid homeostasis in the inner ear for hearing through independent and cooperative mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- OCT4 interprets and enhances nucleosome flexibility.Nucleic acids research · 2022Article
- Evolutionary origin of vertebrate OCT4/POU5 functions in supporting pluripotency.Nature communications · 2022Article
- The functional diversity of the POUV-class proteins across vertebrates.Open biology · 2022Review
- A balanced Oct4 interactome is crucial for maintaining pluripotency.Science advances · 2022Article
- Key features of the POU transcription factor Oct4 from an evolutionary perspective.Cellular and molecular life sciences : CMLS · 2021Review
- Dissecting OCT4 defines the role of nucleosome binding in pluripotency.Nature cell biology · 2021Article
- Hierarchical reactivation of transcription during mitosis-to-G1 transition by Brn2 and Ascl1 in neural stem cells.Genes & development · 2021Article
- Directed Evolution of an Enhanced POU Reprogramming Factor for Cell Fate Engineering.Molecular biology and evolution · 2021Article
- Biological importance of OCT transcription factors in reprogramming and development.Experimental & molecular medicine · 2021Review
- Histone tails cooperate to control the breathing of genomic nucleosomes.PLoS computational biology · 2021Article
- Research progress of the transcription factor Brn4 (Review).Molecular medicine reports · 2021Article
- Article
- Permissive epigenomes endow reprogramming competence to transcriptional regulators.Nature chemical biology · 2021Article
- FOXA1 Mutations Reveal Distinct Chromatin Profiles and Influence Therapeutic Response in Breast Cancer.Cancer cell · 2020Article
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transcription factor Oct4 is a core component of molecular cocktails inducing pluripotent stem cells (iPSCs), while other members of the POU family cannot replace Oct4 with comparable efficiency. Rather, group III POU factors such as Oct6 induce neural lineages. Here, we sought to identify molecular features determining the differential DNA-binding and reprogramming activity of Oct4 and Oct6. In enhancers of pluripotency genes, Oct4 cooperates with Sox2 on heterodimeric SoxOct elements. By re-analyzing ChIP-Seq data and performing dimerization assays, we found that Oct6 homodimerizes on palindromic OctOct more cooperatively and more stably than Oct4. Using structural and biochemical analyses, we identified a single amino acid directing binding to the respective DNA elements. A change in this amino acid decreases the ability of Oct4 to generate iPSCs, while the reverse mutation in Oct6 does not augment its reprogramming activity. Yet, with two additional amino acid exchanges, Oct6 acquires the ability to generate iPSCs and maintain pluripotency. Together, we demonstrate that cell type-specific POU factor function is determined by select residues that affect DNA-dependent dimerization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.