ArticleBiosensors & bioelectronics2017
Automated microraft platform to identify and collect non-adherent cells successfully gene-edited with CRISPR-Cas9.
Article in Biosensors & bioelectronics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The trial behind it
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Who cites it
13 citing papers in PubMed.
- Development of large-scale gastruloid array to identify aberrant developmental phenotypes.APL bioengineering · 2025Article
- Microraft arrays for serial-killer CD19 chimeric antigen receptor T cells and single cell isolation.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2023Article
- Pooled image-base screening of mitochondria with microraft isolation distinguishes pathogenic mitofusin 2 mutations.Communications biology · 2022Article
- Scalable Additive Construction of Arrayed Microstructures with Encoded Properties for Bioimaging.Micromachines · 2022Article
- A technology of a different sort: microraft arrays.Lab on a chip · 2021Review
- Image-Based Live Cell Sorting.Trends in biotechnology · 2021Review
- Review
- Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics.Microfluidics and nanofluidics · 2021Review
- Assay and Isolation of Single Proliferating CD4+ Lymphocytes Using an Automated Microraft Array Platform.IEEE transactions on bio-medical engineering · 2020Article
- Digital Microfluidics for Single Bacteria Capture and Selective Retrieval Using Optical Tweezers.Micromachines · 2020Article
- Automated sensing and splitting of stem cell colonies on microraft arrays.APL bioengineering · 2019Article
- Platforms for Single-Cell Collection and Analysis.International journal of molecular sciences · 2018Review
- A High-Throughput Organoid Microinjection Platform to Study Gastrointestinal Microbiota and Luminal Physiology.Cellular and molecular gastroenterology and hepatology · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Microraft arrays have been used to screen and then isolate adherent and non-adherent cells with very high efficiency and excellent viability; however, manual screening and isolation limits the throughput and utility of the technology. In this work, novel hardware and software were developed to automate the microraft array platform. The developed analysis software identified microrafts on the array with greater than 99% sensitivity and cells on the microrafts with 100% sensitivity. The software enabled time-lapse imaging and the use of temporally varying characteristics as sort criteria. The automated hardware released microrafts with 98% efficiency and collected released microrafts with 100% efficiency. The automated system was used to examine the temporal variation in EGFP expression in cells transfected with CRISPR-Cas9 components for gene editing. Of 11,499 microrafts possessing a single cell, 220 microrafts were identified as possessing temporally varying EGFP-expression. Candidate cells (n=172) were released and collected from the microraft array and screened for the targeted gene mutation. Two cell colonies were successfully gene edited demonstrating the desired mutation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.