ArticlePloS one2016
Unbiased RNA Shotgun Metagenomics in Social and Solitary Wild Bees Detects Associations with Eukaryote Parasites and New Viruses.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 58 citations in OpenAlex.
- Technical advances in robotic retinal surgery: a systematic review and future research directions.Journal of robotic surgery · 2026Review
- Distinct virome compositions and lack of viral diversification indicate that viral spillover is a dead-end between the western honey bee and the common eastern bumblebee.Communications biology · 2025Article
- Honey microbiota, methods for determining the microbiological composition and the antimicrobial effect of honey - A review.Food chemistry: X · 2024Review
- Virome profiling of fig wasps (Ceratosolen spp.) reveals virus diversity spanning four realms.Virology · 2024Article
- A Distinct Arabidopsis Latent Virus 1 Isolate Was Found in WildPlants (Basel, Switzerland) · 2024Article
- Trocar localisation for robot-assisted vitreoretinal surgery.International journal of computer assisted radiology and surgery · 2024Article
- Influence of social lifestyles on host-microbe symbioses in the bees.Ecology and evolution · 2023Article
- RAPIDViruses · 2023Article
- Endosymbionts that threaten commercially raised and wild bumble bees (Journal of pollination ecology · 2023Article
- Ecosystem-specific microbiota and microbiome databases in the era of big data.Environmental microbiome · 2022Review
- Comparative metagenomics reveals expanded insights into intra- and interspecific variation among wild bee microbiomes.Communications biology · 2022Article
- Effects of planted pollinator habitat on pathogen prevalence and interspecific detection between bee species.Scientific reports · 2022Article
- Review
- Virome Analysis Reveals Diverse and Divergent RNA Viruses in Wild Insect Pollinators in Beijing, China.Viruses · 2022Article
- 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales.Archives of virology · 2021Article
- Virus Diversity and Loads in Crickets Reared for Feed: Implications for Husbandry.Frontiers in veterinary science · 2021Article
- Metagenomic Approach with the NetoVIR Enrichment Protocol Reveals Virus Diversity within Ethiopian Honey Bees (Viruses · 2020Article
- Review
- Re-assessing the diversity of negative strand RNA viruses in insects.PLoS pathogens · 2019Article
- Taxonomy of the order Bunyavirales: update 2019.Archives of virology · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diversity of eukaryote organisms and viruses associated with wild bees remains poorly characterized in contrast to the well-documented pathosphere of the western honey bee, Apis mellifera. Using a deliberate RNA shotgun metagenomic sequencing strategy in combination with a dedicated bioinformatics workflow, we identified the (micro-)organisms and viruses associated with two bumble bee hosts, Bombus terrestris and Bombus pascuorum, and two solitary bee hosts, Osmia cornuta and Andrena vaga. Ion Torrent semiconductor sequencing generated approximately 3.8 million high quality reads. The most significant eukaryote associations were two protozoan, Apicystis bombi and Crithidia bombi, and one nematode parasite Sphaerularia bombi in bumble bees. The trypanosome protozoan C. bombi was also found in the solitary bee O. cornuta. Next to the identification of three honey bee viruses Black queen cell virus, Sacbrood virus and Varroa destructor virus-1 and four plant viruses, we describe two novel RNA viruses Scaldis River bee virus (SRBV) and Ganda bee virus (GABV) based on their partial genomic sequences. The novel viruses belong to the class of negative-sense RNA viruses, SRBV is related to the order Mononegavirales whereas GABV is related to the family Bunyaviridae. The potential biological role of both viruses in bees is discussed in the context of recent advances in the field of arthropod viruses. Further, fragmentary sequence evidence for other undescribed viruses is presented, among which a nudivirus in O. cornuta and an unclassified virus related to Chronic bee paralysis virus in B. terrestris. Our findings extend the current knowledge of wild bee parasites in general and addsto the growing evidence of unexplored arthropod viruses in valuable insects.
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