ArticlePloS one2016
Establishment of a 3D In Vitro Model to Accelerate the Development of Human Therapies against Corneal Diabetes.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 26 citations in OpenAlex.
- Fabrication of a 3D Corneal Model Using Collagen Bioink and Human Corneal Stromal Cells.Journal of functional biomaterials · 2025Article
- Novel full-thickness biomimetic corneal model for studying pathogenesis and treatment of diabetic keratopathy.Materials today. Bio · 2025Article
- Corneal epithelial-stromal constructs to study differences associated with diabetes mellitus.Experimental eye research · 2024Article
- Cell sheet-based approach to study the diabetic corneal stroma.Experimental eye research · 2023Article
- The impact of sensory neuropathy and inflammation on epithelial wound healing in diabetic corneas.Progress in retinal and eye research · 2022Review
- Monocarboxylate Transporters: Role and Regulation in Corneal Diabetes.Analytical cellular pathology (Amsterdam) · 2022Article
- FAK Inhibition Attenuates Corneal Fibroblast Differentiation In Vitro.Biomolecules · 2021Article
- Nerve influence on the metabolism of type I and type II diabetic corneal stroma: an in vitro study.Scientific reports · 2021Article
- A novel 3D culture model of fungal keratitis to explore host-pathogen interactions within the stromal environment.Experimental eye research · 2021Article
- The Human Tissue-Engineered Cornea (hTEC): Recent Progress.International journal of molecular sciences · 2021Review
- Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye.Pharmaceutics · 2020Review
- Methods for Investigating Corneal Cell Interactions and Extracellular Vesicles In Vitro.Current protocols in cell biology · 2020Article
- Modeling the cornea in 3-dimensions: Current and future perspectives.Experimental eye research · 2020Review
- Pathogenic Role of PPARα Downregulation in Corneal Nerve Degeneration and Impaired Corneal Sensitivity in Diabetes.Diabetes · 2020Article
- Review
- Association between Diabetes and Keratoconus: A Retrospective Analysis.Scientific reports · 2019Article
- Modeling Diabetic Corneal Neuropathy in a 3D In Vitro Cornea System.Scientific reports · 2018Article
- Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea.Journal of visualized experiments : JoVE · 2018Article
- 3D Stacked Construct: A Novel Substitute for Corneal Tissue Engineering.Methods in molecular biology (Clifton, N.J.) · 2018Article
- The role of lipids in corneal diseases and dystrophies: a systematic review.Clinical and translational medicine · 2017Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
purposeTo establish an in vitro model that would mirror the in vivo corneal stromal environment in diabetes (DM) patients.
methodsHuman corneal fibroblasts from Healthy (HCFs), Type 1DM (T1DM) and Type 2DM (T2DM) donors were isolated and cultured for 4 weeks with Vitamin C stimulation in order to allow for extracellular matrix (ECM) secretion and assembly.
resultsOur data indicated altered cellular morphology, increased cellular migration, increased ECM assembly, and severe mitochondrial damage in both T1DM and T2DMs when compared to HCFs. Furthermore, we found significant downregulation of Collagen I and Collagen V expression in both T1DM and T2DMs. Furthermore, a significant up regulation of fibrotic markers was seen, including α-smooth muscle actin in T2DM and Collagen III in both T1DM and T2DMs. Metabolic analysis suggested impaired Glycolysis and Tricarboxylic acid cycle (TCA) pathway.
conclusionDM has significant effects on physiological and clinical aspects of the human cornea. The benefits in developing and fully characterizing our 3D in vitro model are enormous and might provide clues for the development of novel therapeutics.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.