ArticlePloS one2016
Norlichexanthone Reduces Virulence Gene Expression and Biofilm Formation in Staphylococcus aureus.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
- DisarmingPathogens (Basel, Switzerland) · 2025Review
- Lichen and Its Microbiome as an Untapped Source of Anti-Biofilm Compounds.Chemistry & biodiversity · 2025Review
- Recent advances in Polygonaceae endophytes: diversity, secondary metabolites and biotechnological applications.Frontiers in bioengineering and biotechnology · 2025Review
- Forssman and the staphylococcal hemolysins.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2025Review
- Article
- Inhibition of Staphylococcus aureus Biofilm Formation and Virulence Factor Production by Petroselinic Acid and Other Unsaturated C18 Fatty Acids.Microbiology spectrum · 2022Article
- Loratadine inhibitsiScience · 2022Article
- Antibiofilm Activity of Phorbaketals from the Marine SpongeMarine drugs · 2021Article
- Bacterial Targets of Antibiotics in Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2021Review
- A Novel Aza-Derivative InhibitsFrontiers in microbiology · 2021Article
- Synthesis of a Small Library of Nature-Inspired Xanthones and Study of Their Antimicrobial Activity.Molecules (Basel, Switzerland) · 2020Article
- Quorum Sensing as Antivirulence Target in Cystic Fibrosis Pathogens.International journal of molecular sciences · 2019Review
- Antibiotic Resistance and the MRSA Problem.Microbiology spectrum · 2019Review
- Effect of Co-inhabiting Coagulase Negative Staphylococci onFrontiers in microbiology · 2019Article
- Transcriptional Analysis of the Effects of Gambogic Acid and Neogambogic Acid on Methicillin-ResistantFrontiers in pharmacology · 2019Article
- Diversity and Ecology of Marine Algicolous Arthrinium Species as a Source of Bioactive Natural Products.Marine drugs · 2018Article
- Total Synthesis of Xanthoangelol B and Its Various Fragments: Toward Inhibition of Virulence Factor Production of Staphylococcus aureus.Journal of medicinal chemistry · 2018Article
- Innovative drugs, chemicals, and enzymes within the animal production chain.Veterinary research · 2018Review
- Linear peptidomimetics as potent antagonists of Staphylococcus aureus agr quorum sensing.Scientific reports · 2018Article
- Interference With Quorum-Sensing Signal Biosynthesis as a Promising Therapeutic Strategy Against Multidrug-Resistant Pathogens.Frontiers in cellular and infection microbiology · 2018Review
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Staphylococcus aureus is a serious human pathogen and antibiotic resistant, community-associated strains, such as the methicillin resistant S. aureus (MRSA) strain USA300, continue to spread. To avoid resistance, anti-virulence therapy has been proposed where toxicity is targeted rather than viability. Previously we have shown that norlichexanthone, a small non-reduced tricyclic polyketide produced by fungi and lichens, reduces expression of hla encoding α-hemolysin as well as the regulatory RNAIII of the agr quorum sensing system in S. aureus 8325-4. The aim of the present study was to further characterise the mode of action of norlichexanthone and its effect on biofilm formation. We find that norlichexanthone reduces expression of both hla and RNAIII also in strain USA300. Structurally, norlichexanthone resembles ω-hydroxyemodin that recently was shown to bind the agr two component response regulator, AgrA, which controls expression of RNAIII and the phenol soluble modulins responsible for human neutrophil killing. We show that norlichexanthone reduces S. aureus toxicity towards human neutrophils and interferes directly with AgrA binding to its DNA target. In contrast to ω-hydroxyemodin however, norlichexanthone reduces staphylococcal biofilm formation. Transcriptomic analysis revealed that genes regulated by the SaeRS two-component system are repressed by norlichexanthone when compared to untreated cells, an effect that was mitigated in strain Newman carrying a partially constitutive SaeRS system. Our data show that norlichexanthone treatment reduces expression of key virulence factors in CA-MRSA strain USA300 via AgrA binding and represses biofilm formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.