ArticleRNA biology2017
Defining the purity of exosomes required for diagnostic profiling of small RNA suitable for biomarker discovery.
Article in RNA biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 44 citations in OpenAlex.
- Molecular Evaluation of Different Enrichment Methods for Extracellular Vesicles from Healthy Subjects' Biobanked Serum.International journal of molecular sciences · 2026Article
- Standardized Reporting of Research on Exosomes to Ensure Rigor and Reproducibility.Advances in wound care · 2024Review
- Temporal changes in mouse hippocampus transcriptome after pilocarpine-induced seizures.Frontiers in neuroscience · 2024Article
- Identification of microRNA biomarkers simultaneously expressed in circulating extracellular vesicles and atherosclerotic plaques.Frontiers in cardiovascular medicine · 2024Article
- Exosomes and ultrasound: The future of theranostic applications.Materials today. Bio · 2023Review
- Long Non-Coding RNAs (lncRNAs) as Regulators of the PI3K/AKT/mTOR Pathway in Gastric Carcinoma.International journal of molecular sciences · 2023Review
- An update on our understanding of Gram-positive bacterial membrane vesicles: discovery, functions, and applications.Frontiers in cellular and infection microbiology · 2023Review
- piRNAQuest V.2: an updated resource for searching through the piRNAome of multiple species.RNA biology · 2022Article
- Exosomal Composition, Biogenesis and Profiling Using Point-of-Care Diagnostics-Implications for Cardiovascular Disease.Frontiers in cell and developmental biology · 2022Review
- Conventional and Nonconventional Sources of Exosomes-Isolation Methods and Influence on Their Downstream Biomedical Application.Frontiers in molecular biosciences · 2022Review
- The Emerging Role of PIWI-Interacting RNAs (piRNAs) in Gastrointestinal Cancers: An Updated Perspective.Cancers · 2021Review
- Let-7b-5p in vesicles secreted by human airway cells reduces biofilm formation and increases antibiotic sensitivity ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Understanding extracellular vesicle and nanoparticle heterogeneity: Novel methods and considerations.Proteomics · 2021Review
- Circulating Exosomes of Neuronal Origin as Potential Early Biomarkers for Development of Stroke.Molecular diagnosis & therapy · 2021Review
- Cancer-secreted miRNAs regulate amino-acid-induced mTORC1 signaling and fibroblast protein synthesis.EMBO reports · 2021Article
- Review
- Extracellular Vesicle Associated miRNAs Regulate Signaling Pathways Involved in COVID-19 Pneumonia and the Progression to Severe Acute Respiratory Corona Virus-2 Syndrome.Frontiers in immunology · 2021Article
- An intact membrane is essential for small extracellular vesicle-induced modulation of α-synuclein fibrillization.Journal of extracellular vesicles · 2020Article
- Small RNA fingerprinting of Alzheimer's disease frontal cortex extracellular vesicles and their comparison with peripheral extracellular vesicles.Journal of extracellular vesicles · 2020Article
- Characterization of exosomes in peritoneal fluid of endometriosis patients.Fertility and sterility · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small non-coding RNAs (ncRNA), including microRNAs (miRNA), enclosed in exosomes are being utilised for biomarker discovery in disease. Two common exosome isolation methods involve differential ultracentrifugation or differential ultracentrifugation coupled with Optiprep gradient fractionation. Generally, the incorporation of an Optiprep gradient provides better separation and increased purity of exosomes. The question of whether increased purity of exosomes is required for small ncRNA profiling, particularly in diagnostic and biomarker purposes, has not been addressed and highly debated. Utilizing an established neuronal cell system, we used next-generation sequencing to comprehensively profile ncRNA in cells and exosomes isolated by these 2 isolation methods. By comparing ncRNA content in exosomes from these two methods, we found that exosomes from both isolation methods were enriched with miRNAs and contained a diverse range of rRNA, small nuclear RNA, small nucleolar RNA and piwi-interacting RNA as compared with their cellular counterparts. Additionally, tRNA fragments (30-55 nucleotides in length) were identified in exosomes and may act as potential modulators for repressing protein translation. Overall, the outcome of this study confirms that ultracentrifugation-based method as a feasible approach to identify ncRNA biomarkers in exosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.