ArticleACS chemical biology2017
A Rationally Designed, General Strategy for Membrane Orientation of Photoinduced Electron Transfer-Based Voltage-Sensitive Dyes.
Article in ACS chemical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Electric field-dependent excited-state twisting in donor-acceptor thiazolothiazole dyes establishes a new class of cell membrane voltage sensors.Chemical science · 2026Article
- Sub-millivolt voltage imaging reveals gap junction-mediated bioelectric contact inhibition.Nature communications · 2026Article
- Controlling Electron Flow in Carbofluorescein Voltage Indicators.ACS chemical biology · 2025Article
- Towards measurements of absolute membrane potential in Bacillus subtilis using fluorescence lifetime.bioRxiv : the preprint server for biology · 2024Article
- Extended voltage imaging in cardiomyocytes with a triplet state quencher-stabilized silicon rhodamine.Bioorganic & medicinal chemistry letters · 2024Article
- An open and shut case? Chemistry to control xanthene dyes.Trends in chemistry · 2024Article
- Orientation and Membrane Partition Free Energy of PeT-Based Voltage-Sensitive Dyes from Molecular Simulations.The journal of physical chemistry. B · 2024Article
- Leveraging Coupled Solvatofluorochromism and Fluorescence Quenching in Nitrophenyl-Containing Thiazolothiazoles for Efficient Organic Vapor Sensing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Fluorescence Imaging of Cell Membrane Potential: From Relative Changes to Absolute Values.International journal of molecular sciences · 2023Review
- Electrically controlling and optically observing the membrane potential of supported lipid bilayers.Biophysical journal · 2022Article
- Chemical Targeting of Rhodol Voltage-Sensitive Dyes to Dopaminergic Neurons.ACS chemical neuroscience · 2022Article
- Lessons in Organic Fluorescent Probe Discovery.Chembiochem : a European journal of chemical biology · 2021Review
- Mechanistic Understanding from Molecular Dynamics in Pharmaceutical Research 2: Lipid Membrane in Drug Design.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Vinyl-Fluorene Molecular Wires for Voltage Imaging with Enhanced Sensitivity and Reduced Phototoxicity.Journal of the American Chemical Society · 2021Article
- Phosphonofluoresceins: Synthesis, Spectroscopy, and Applications.Journal of the American Chemical Society · 2021Article
- Voltage Imaging with a NIR-Absorbing Phosphine Oxide Rhodamine Voltage Reporter.Journal of the American Chemical Society · 2021Article
- Fluorescence lifetime predicts performance of voltage sensitive fluorophores in cardiomyocytes and neurons.RSC chemical biology · 2021Article
- Optical Spike Detection and Connectivity Analysis With a Far-Red Voltage-Sensitive Fluorophore Reveals Changes to Network Connectivity in Development and Disease.Frontiers in neuroscience · 2021Article
- Therapeutic Antibodies Targeting Potassium Ion Channels.Handbook of experimental pharmacology · 2021Article
- The Secret Lives of Fluorescent Membrane Probes as Revealed by Molecular Dynamics Simulations.Molecules (Basel, Switzerland) · 2020Review
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Authors and funding
8 authors.
Funding
Abstract
Voltage imaging with fluorescent dyes offers promise for interrogating the complex roles of membrane potential in coordinating the activity of neurons in the brain. Yet, low sensitivity often limits the broad applicability of optical voltage indicators. In this paper, we use molecular dynamics (MD) simulations to guide the design of new, ultrasensitive fluorescent voltage indicators that use photoinduced electron transfer (PeT) as a voltage-sensing switch. MD simulations predict an approximately 16% increase in voltage sensitivity resulting purely from improved alignment of dye with the membrane. We confirm this theoretical finding by synthesizing 9 new voltage-sensitive (VoltageFluor, or VF) dyes and establishing that all of them display the expected improvement of approximately 19%. This synergistic outworking of theory and experiment enabled computational and theoretical estimation of VF dye orientation in lipid bilayers and has yielded the most sensitive PeT-based VF dye to date. We use this new voltage indicator to monitor voltage spikes in neurons from rat hippocampus and human pluripotent-stem-cell-derived dopaminergic neurons.
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