ArticleMicrobial biotechnology2017
Novel thermostable antibiotic resistance enzymes from the Atlantis II Deep Red Sea brine pool.
Article in Microbial biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Microbial dark matter spearheading the biogeochemical cycle in the Solar Lake of Taba, Egypt.Current research in microbial sciences · 2025Article
- Precision enzyme discovery through targeted mining of metagenomic data.Natural products and bioprospecting · 2024Review
- Assessment of genetically modified cotton COT102 for food and feed uses, under Regulation (EC) No 1829/2003 (application EFSA-GMO-DE-2017-141).EFSA journal. European Food Safety Authority · 2023Article
- Metagenomic profiling of antibiotic resistance genes in Red Sea brine pools.Archives of microbiology · 2023Article
- β-Lactams from the Ocean.Marine drugs · 2023Review
- Novel Enzymes From the Red Sea Brine Pools: Current State and Potential.Frontiers in microbiology · 2021Review
- Freshwater viral metagenome reveals novel and functional phage-borne antibiotic resistance genes.Microbiome · 2020Article
- Current Trends in Experimental and Computational Approaches to Combat Antimicrobial Resistance.Frontiers in genetics · 2020Review
- Insights into Red Sea Brine Pool Specialized Metabolism Gene Clusters Encoding Potential Metabolites for Biotechnological Applications and Extremophile Survival.Marine drugs · 2019Article
- Antibacterial and anticancer activities of orphan biosynthetic gene clusters from Atlantis II Red Sea brine pool.Microbial cell factories · 2019Article
- Genetic Tools and Techniques for Recombinant Expression in Thermophilic Bacillaceae.Microorganisms · 2018Review
- Antibiotic resistance in the most unlikeliest of places.Microbial biotechnology · 2017Article
- Novel thermostable antibiotic resistance enzymes from the Atlantis II Deep Red Sea brine pool.Microbial biotechnology · 2017Article
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Authors and funding
3 authors.
Funding
Abstract
The advent of metagenomics has greatly facilitated the discovery of enzymes with useful biochemical characteristics for industrial and biomedical applications, from environmental niches. In this study, we used sequence-based metagenomics to identify two antibiotic resistance enzymes from the secluded, lower convective layer of Atlantis II Deep Red Sea brine pool (68°C, ~2200 m depth and 250‰ salinity). We assembled > 4 000 000 metagenomic reads, producing 43 555 contigs. Open reading frames (ORFs) called from these contigs were aligned to polypeptides from the Comprehensive Antibiotic Resistance Database using BLASTX. Two ORFs were selected for further analysis. The ORFs putatively coded for 3'-aminoglycoside phosphotransferase [APH(3')] and a class A beta-lactamase (ABL). Both genes were cloned, expressed and characterized for activity and thermal stability. Both enzymes were active in vitro, while only APH(3') was active in vivo. Interestingly, APH(3') proved to be thermostable (T
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