ArticleScientific reports2016
3-D Microwell Array System for Culturing Virus Infected Tumor Cells.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Sound-based assembly of three-dimensional cellularized and acellularized constructs.Materials today. Bio · 2023Article
- Three-Dimensional Culture ofInsects · 2021Article
- Tumor-on-a-chip for integrating a 3D tumor microenvironment: chemical and mechanical factors.Lab on a chip · 2020Review
- Microfluidic technologies to engineer mesenchymal stem cell aggregates-applications and benefits.Biophysical reviews · 2020Review
- Facile Method for Fabricating Microfluidic Chip Integrated with Microwell Arrays for Cell Trapping.Micromachines · 2019Article
- A Multiwell Microfluidic Device for Analyzing and Screening Nonhormonal Contraceptive Agents.Small (Weinheim an der Bergstrasse, Germany) · 2019Article
- An endothelial cell line infected by Kaposi's sarcoma-associated herpes virus (KSHV) allows the investigation of Kaposi's sarcoma and the validation of novel viral inhibitors in vitro and in vivo.Journal of molecular medicine (Berlin, Germany) · 2019Article
- Article
- miRNA-36 inhibits KSHV, EBV, HSV-2 infection of cells via stifling expression of interferon induced transmembrane protein 1 (IFITM1).Scientific reports · 2017Article
- Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment.Chemical reviews · 2017Review
- Monitoring Neutropenia for Cancer Patients at the Point of Care.Small methods · 2017Article
- Creating artificial lymphoid tissues to study immunity and hematological malignancies.Current opinion in hematology · 2017Review
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
Cancer cells have been increasingly grown in pharmaceutical research to understand tumorigenesis and develop new therapeutic drugs. Currently, cells are typically grown using two-dimensional (2-D) cell culture approaches, where the native tumor microenvironment is difficult to recapitulate. Thus, one of the main obstacles in oncology is the lack of proper infection models that recount main features present in tumors. In recent years, microtechnology-based platforms have been employed to generate three-dimensional (3-D) models that better mimic the native microenvironment in cell culture. Here, we present an innovative approach to culture Kaposi's sarcoma-associated herpesvirus (KSHV) infected human B cells in 3-D using a microwell array system. The results demonstrate that the KSHV-infected B cells can be grown up to 15 days in a 3-D culture. Compared with 2-D, cells grown in 3-D had increased numbers of KSHV latency-associated nuclear antigen (LANA) dots, as detected by immunofluorescence microscopy, indicating a higher viral genome copy number. Cells in 3-D also demonstrated a higher rate of lytic reactivation. The 3-D microwell array system has the potential to improve 3-D cell oncology models and allow for better-controlled studies for drug discovery.
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