ArticleScientific reports2016
Acute transcriptional up-regulation specific to osteoblasts/osteoclasts in medaka fish immediately after exposure to microgravity.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- Microgravity-Related Changes in Bone Density and Treatment Options: A Systematic Review.International journal of molecular sciences · 2022Pooled it
- Multi-Omics Characterization of Human Molecular Responses to Spaceflight Across Two Independent Missions.bioRxiv : the preprint server for biology · 2026Article
- Impact of microgravity and lunar gravity on murine skeletal and immune systems during space travel.Scientific reports · 2024Article
- Microgravity and Musculoskeletal Health: What Strategies Should Be Used for a Great Challenge?Life (Basel, Switzerland) · 2023Review
- Microgravity enhances the phenotype of Arabidopsis zigzag-1 and reduces the Wortmannin-induced vacuole fusion in root cells.NPJ microgravity · 2022Article
- Real and Simulated Microgravity: Focus on Mammalian Extracellular Matrix.Life (Basel, Switzerland) · 2022Review
- The effects of microgravity on bone structure and function.NPJ microgravity · 2022Review
- Effects of Mechanical Stress Stimulation on Function and Expression Mechanism of Osteoblasts.Frontiers in bioengineering and biotechnology · 2022Review
- Microgravity Effects on the Matrisome.Cells · 2021Review
- Update on the effects of microgravity on the musculoskeletal system.NPJ microgravity · 2021Review
- Findings from recent studies by the Japan Aerospace Exploration Agency examining musculoskeletal atrophy in space and on Earth.NPJ microgravity · 2021Review
- The regulation of RANKL by mechanical force.Journal of bone and mineral metabolism · 2021Review
- Fish Models of Induced Osteoporosis.Frontiers in cell and developmental biology · 2021Review
- Depression of Bone Density at the Weight-Bearing Joints in Wistar Hannover Rats by a Simulated Mechanical Stress Associated With Partial Gravity Environment.Frontiers in cell and developmental biology · 2021Article
- Influence of Microgravity on Apoptosis in Cells, Tissues, and Other Systems In Vivo and In Vitro.International journal of molecular sciences · 2020Review
- Osteoclasts and Microgravity.Life (Basel, Switzerland) · 2020Review
- Reptiles in Space Missions: Results and Perspectives.International journal of molecular sciences · 2019Review
- Gene Expression in Osteoblasts and Osteoclasts Under Microgravity Conditions: A Systematic Review.Current genomics · 2019Review
- Deep Transcriptomic Analysis of Black Rockfish (Sebastes schlegelii) Provides New Insights on Responses to Acute Temperature Stress.Scientific reports · 2018Article
- Biofabrication of in situ Self Assembled 3D Cell Cultures in a Weightlessness Environment Generated using Magnetic Levitation.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
15 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bone loss is a serious problem in spaceflight; however, the initial action of microgravity has not been identified. To examine this action, we performed live-imaging of animals during a space mission followed by transcriptome analysis using medaka transgenic lines expressing osteoblast and osteoclast-specific promoter-driven GFP and DsRed. In live-imaging for osteoblasts, the intensity of osterix- or osteocalcin-DsRed fluorescence in pharyngeal bones was significantly enhanced 1 day after launch; and this enhancement continued for 8 or 5 days. In osteoclasts, the signals of TRAP-GFP and MMP9-DsRed were highly increased at days 4 and 6 after launch in flight. HiSeq from pharyngeal bones of juvenile fish at day 2 after launch showed up-regulation of 2 osteoblast- and 3 osteoclast- related genes. Gene ontology analysis for the whole-body showed that transcription of genes in the category "nucleus" was significantly enhanced; particularly, transcription-regulators were more up-regulated at day 2 than at day 6. Lastly, we identified 5 genes, c-fos, jun-B-like, pai-1, ddit4 and tsc22d3, which were up-regulated commonly in the whole-body at days 2 and 6, and in the pharyngeal bone at day 2. Our results suggested that exposure to microgravity immediately induced dynamic alteration of gene expression levels in osteoblasts and osteoclasts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.