ArticleNature communications2016
Proximity does not contribute to activity enhancement in the glucose oxidase-horseradish peroxidase cascade.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
76 citing papers in PubMed.
- Confined Catalysis of Enzymes: Enhancement Strategies, Materials, and Biosensor Applications.Journal of functional biomaterials · 2026Review
- DNA-Enzyme Hybrid Nanostructures: Functional Materials to Modulate Enzymatic Activity.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Complete enzyme clustering enhances coenzyme Q biosynthesis via substrate channeling.Nature communications · 2026Article
- Stoichiometrically Engineered Hydrated Ionic Liquids Enabling Reinforcement of Enzyme Cascade with Improved Thermodynamic Stability.ACS sustainable chemistry & engineering · 2026Article
- Carrier-Free Enzyme Immobilization for High-Density Catalytic Architectures in Flow Biocatalysis.JACS Au · 2026Review
- Engineering Artificial Mitochondria with Self-Amplifying Proton Generation for Autonomous Energy Supply and Metabolic Coupling in Artificial Cells.Angewandte Chemie (International ed. in English) · 2025Article
- Article
- Dynamic Hydrogel-Based Cytomimetic Models for Chemical Information Conduction.Small science · 2025Article
- Noncovalently Immobilized Glucose Oxidase/Horseradish Peroxidase Cascade on Polyamide Supports for Eco-Friendly Polyaniline Synthesis.Molecules (Basel, Switzerland) · 2025Article
- Electrochemical Investigation of Enzyme Kinetics with an Unmediated, Unmodified Platinum Microelectrode: The Case of Glucose Oxidase.ACS electrochemistry · 2025Article
- Enzymes helping enzymes: Oxaloacetate decarboxylase increases malate dehydrogenase's turnover number.PNAS nexus · 2025Article
- Genetically engineered secretory horseradish peroxidase is a sensitive, stable, and affordable non-lytic reporter gene system for real-time promoter activity management.Journal of biological engineering · 2025Article
- Spontaneous Hollow Coacervate Transition of Silk Fibroin via Dilution and Its Transition to Microcapsules.Biomacromolecules · 2025Article
- Enzyme Reactions Are Accelerated or Decelerated When the Enzymes Are Located Near the DNA Nanostructure.ACS applied materials & interfaces · 2025Article
- Assembly and biological functions of metal-biomolecule network nanoparticles formed by metal-phosphonate coordination.Science advances · 2024Article
- Organ-Specific Gene Expression Control Using DNA Origami-Based Nanodevices.Nano letters · 2024Article
- Overview on the Development of Electrochemical Immunosensors by the Signal Amplification of Enzyme- or Nanozyme-Based Catalysis Plus Redox Cycling.Molecules (Basel, Switzerland) · 2024Review
- Interactive biocatalysis achieved by driving enzyme cascades inside a porous conducting material.Communications chemistry · 2024Review
- Immunobiology of cancer stem cells and their immunoevasion mechanisms.Molecular biology reports · 2023Review
- Acid-resistant enzymes: the acquisition strategies and applications.Applied microbiology and biotechnology · 2023Review
16 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A proximity effect has been invoked to explain the enhanced activity of enzyme cascades on DNA scaffolds. Using the cascade reaction carried out by glucose oxidase and horseradish peroxidase as a model system, here we study the kinetics of the cascade reaction when the enzymes are free in solution, when they are conjugated to each other and when a competing enzyme is present. No proximity effect is found, which is in agreement with models predicting that the rapidly diffusing hydrogen peroxide intermediate is well mixed. We suggest that the reason for the activity enhancement of enzymes localized by DNA scaffolds is that the pH near the surface of the negatively charged DNA nanostructures is lower than that in the bulk solution, creating a more optimal pH environment for the anchored enzymes. Our findings challenge the notion of a proximity effect and provide new insights into the role of DNA scaffolds.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.