ArticleMetabolomics : Official journal of the Metabolomic Society2016
A novel targeted/untargeted GC-Orbitrap metabolomics methodology applied to
Article in Metabolomics : Official journal of the Metabolomic Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 46 citations in OpenAlex.
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- LC-HRMS/MS-Based Metabolomics Approaches Applied to the Detection of Antifungal Compounds and a Metabolic Dynamic Assessment of Orchidaceae.Molecules (Basel, Switzerland) · 2022Article
- A high-resolution Orbitrap Mass spectral library for trace volatile compounds in fruit wines.Scientific data · 2022Article
- Advances in Plant Metabolomics and Its Applications in Stress and Single-Cell Biology.International journal of molecular sciences · 2022Review
- Characterization of Volatile Profiles and Correlated Contributing Compounds in Pan-Fried Steaks from Different Chinese Yellow Cattle Breeds through GC-Q-Orbitrap, E-Nose, and Sensory Evaluation.Molecules (Basel, Switzerland) · 2022Article
- Advances in experimental and computational methodologies for the study of microbial-surface interactions at different omics levels.Frontiers in microbiology · 2022Review
- Optimization of metabolomic data processing using NOREVA.Nature protocols · 2022Review
- Practical Considerations in Method Development for Gas Chromatography-Based Metabolomic Profiling.Advances in experimental medicine and biology · 2021Article
- A two-step gas chromatography-tandem mass spectrometry method for measurement of multiple environmental pollutants in human plasma.Environmental science and pollution research international · 2021Article
- Open, High-Resolution EI+ Spectral Library of Anthropogenic Compounds.Frontiers in public health · 2021Article
- Catch the wave: Metabolomic analyses in human pathogenic fungi.PLoS pathogens · 2020Article
- High Resolution GC-Orbitrap-MS Metabolomics Using Both Electron Ionization and Chemical Ionization for Analysis of Human Plasma.Journal of proteome research · 2020Article
- NOREVA: enhanced normalization and evaluation of time-course and multi-class metabolomic data.Nucleic acids research · 2020Article
- Metabolomics Benefits from Orbitrap GC-MS-Comparison of Low- and High-Resolution GC-MS.Metabolites · 2020Article
- Analysing central metabolism in ultra-high resolution: At the crossroads of carbon and nitrogen.Molecular metabolism · 2020Review
- High-resolution gas chromatography/mass spectrometry metabolomics of non-human primate serum.Rapid communications in mass spectrometry : RCM · 2018Article
- Gas Chromatography-Tandem Mass Spectrometry of Lignin Pyrolyzates with Dopant-Assisted Atmospheric Pressure Chemical Ionization and Molecular Structure Search with CSI:FingerID.Journal of the American Society for Mass Spectrometry · 2018Article
- Review: Endogenously Produced Volatiles forApplied in vitro toxicology · 2018Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
introductionCombined infections from
objectivesTo explore the interaction, we used a novel GC-Orbitrap-based mass spectrometer, the Q Exactive GC, which combines the high peak capacity and chromatographic resolution of gas chromatography with the sub-ppm mass accuracy of an Orbitrap system. This allows the capability to leverage the widely available electron ionisation libraries for untargeted applications, along with expanding accurate mass libraries and targeted matches based around authentic standards.
methodsOptimised
resultsThe targeted analysis experiment was based around 36 sugars and sugar phosphates, 22 amino acids and five organic acids. Untargeted analysis resulted in the detection of 465 features from fresh and spent medium and 405 from biofilm samples. Three significantly changing compounds that matched to high scoring library fragment patterns were chosen for validation.
conclusionEvaluation of the results demonstrates that the Q Exactive GC is suitable for metabolomics analysis using a targeted/untargeted methodology. Many of the results were as expected: e.g. rapid consumption of glucose and fructose from the medium regardless of the cell type. Modulation of sugar-phosphate levels also suggest that the pentose phosphate pathway could be enhanced in the cells from co-cultured biofilms. Untargeted metabolomics results suggested significant production of cell-wall biosynthesis components and the consumption of non-proteinaceous amino-acids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.