ArticleNucleic acids research2017
Single cell transcriptomics reveals unanticipated features of early hematopoietic precursors.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 49 citations in OpenAlex.
- Cell-Type-Specific Calibration of Mitochondrial Ubiquitination in Stem Cell Fate Decisions.Stem cell reviews and reports · 2026Review
- Innate immune mechanisms hijacked by leukemia-initiating stem cells for selective advantage and immune evasion in Ptpn11-associated JMML.Cell reports · 2025Article
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- StemDriver: a knowledgebase of gene functions for hematopoietic stem cell fate determination.Nucleic acids research · 2024Article
- IndepthPathway: an integrated tool for in-depth pathway enrichment analysis based on single-cell sequencing data.Bioinformatics (Oxford, England) · 2023Article
- ABC portal: a single-cell database and web server for blood cells.Nucleic acids research · 2023Article
- The role of serum amyloid A1 in the adipogenic differentiation of human adipose-derived stem cells basing on single-cell RNA sequencing analysis.Stem cell research & therapy · 2022Article
- Tumorigenicity risk of iPSCsPrecision clinical medicine · 2022Review
- SkewC: Identifying cells with skewed gene body coverage in single-cell RNA sequencing data.iScience · 2022Article
- Article
- Cell size is a determinant of stem cell potential during aging.Science advances · 2021Article
- Spatial transcriptome profiling by MERFISH reveals fetal liver hematopoietic stem cell niche architecture.Cell discovery · 2021Article
- Article
- Comprehensive analysis of single-cell RNA sequencing data from healthy human marrow hematopoietic cells.BMC research notes · 2020Article
- Universal concept signature analysis: genome-wide quantification of new biological and pathological functions of genes and pathways.Briefings in bioinformatics · 2020Article
- Spatial-omics: Novel approaches to probe cell heterogeneity and extracellular matrix biology.Matrix biology : journal of the International Society for Matrix Biology · 2020Review
- The Polycomb-associated factor PHF19 controls hematopoietic stem cell state and differentiation.Science advances · 2020Article
- Transcriptomics in Toxicogenomics, Part I: Experimental Design, Technologies, Publicly Available Data, and Regulatory Aspects.Nanomaterials (Basel, Switzerland) · 2020Review
- CHETAH: a selective, hierarchical cell type identification method for single-cell RNA sequencing.Nucleic acids research · 2019Article
- Chasing Mavericks: The quest for defining developmental waves of hematopoiesis.Current topics in developmental biology · 2019Review
Corrections and comments
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Authors and funding
16 authors at 8 institutions in 2 countries.
Funding
Abstract
Molecular changes underlying stem cell differentiation are of fundamental interest. scRNA-seq on murine hematopoietic stem cells (HSC) and their progeny MPP1 separated the cells into 3 main clusters with distinct features: active, quiescent, and an un-characterized cluster. Induction of anemia resulted in mobilization of the quiescent to the active cluster and of the early to later stage of cell cycle, with marked increase in expression of certain transcription factors (TFs) while maintaining expression of interferon response genes. Cells with surface markers of long term HSC increased the expression of a group of TFs expressed highly in normal cycling MPP1 cells. However, at least Id1 and Hes1 were significantly activated in both HSC and MPP1 cells in anemic mice. Lineage-specific genes were differently expressed between cells, and correlated with the cell cycle stages with a specific augmentation of erythroid related genes in the G2/M phase. Most lineage specific TFs were stochastically expressed in the early precursor cells, but a few, such as Klf1, were detected only at very low levels in few precursor cells. The activation of these factors may correlate with stages of differentiation. This study reveals effects of cell cycle progression on the expression of lineage specific genes in precursor cells, and suggests that hematopoietic stress changes the balance of renewal and differentiation in these homeostatic cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.