ArticleProceedings of the National Academy of Sciences of the United States of America2017
Whole-GUV patch-clamping.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 55 citations in OpenAlex.
- Giant unilamellar vesicles as a model system for studying ion transport.Biophysical reviews · 2025Review
- Sterols in plant biologyCell surface (Amsterdam, Netherlands) · 2025Review
- Live-cell quantification reveals viscoelastic regulation of synapsin condensates by α-synuclein.Science advances · 2025Article
- Membrane Transport Modulates the pH-Regulated Feedback of an Enzyme Reaction Confined within Lipid Vesicles.ACS nano · 2025Article
- A simple method to make, trap and deform a vesicle in a gel.Scientific reports · 2023Article
- Freestanding lipid bilayer tensiometer for the study of mechanosensitive ion channels.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Regulation of membrane protein structure and function by their lipid nano-environment.Nature reviews. Molecular cell biology · 2023Review
- Assessing membrane material properties from the response of giant unilamellar vesicles to electric fields.Advances in physics: X · 2023Article
- Article
- [Study on the effect of small alcohol on lipid hydration and liposome formation].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2022Article
- Article
- Investigating the structural properties of hydrophobic solvent-rich lipid bilayers.Soft matter · 2021Article
- PoET: automated approach for measuring pore edge tension in giant unilamellar vesicles.Bioinformatics advances · 2021Article
- Cell-Derived Plasma Membrane Vesicles Are Permeable to Hydrophilic Macromolecules.Biophysical journal · 2020Article
- Combining patch-clamping and fluorescence microscopy for quantitative reconstitution of cellular membrane processes with Giant Suspended Bilayers.Scientific reports · 2019Article
- EXP2 is a nutrient-permeable channel in the vacuolar membrane of Plasmodium and is essential for protein export via PTEX.Nature microbiology · 2018Article
- Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients.Journal of visualized experiments : JoVE · 2018Article
- Dependence and Homeostasis of Membrane Impedance on Cell Morphology in Cultured Hippocampal Neurons.Scientific reports · 2018Article
- Mechanical Transduction and the Dark Energy of Biology.Biophysical journal · 2018Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studying how the membrane modulates ion channel and transporter activity is challenging because cells actively regulate membrane properties, whereas existing in vitro systems have limitations, such as residual solvent and unphysiologically high membrane tension. Cell-sized giant unilamellar vesicles (GUVs) would be ideal for in vitro electrophysiology, but efforts to measure the membrane current of intact GUVs have been unsuccessful. In this work, two challenges for obtaining the "whole-GUV" patch-clamp configuration were identified and resolved. First, unless the patch pipette and GUV pressures are precisely matched in the GUV-attached configuration, breaking the patch membrane also ruptures the GUV. Second, GUVs shrink irreversibly because the membrane/glass adhesion creating the high-resistance seal (>1 GΩ) continuously pulls membrane into the pipette. In contrast, for cell-derived giant plasma membrane vesicles (GPMVs), breaking the patch membrane allows the GPMV contents to passivate the pipette surface, thereby dynamically blocking membrane spreading in the whole-GMPV mode. To mimic this dynamic passivation mechanism, beta-casein was encapsulated into GUVs, yielding a stable, high-resistance, whole-GUV configuration for a range of membrane compositions. Specific membrane capacitance measurements confirmed that the membranes were truly solvent-free and that membrane tension could be controlled over a physiological range. Finally, the potential for ion transport studies was tested using the model ion channel, gramicidin, and voltage-clamp fluorometry measurements were performed with a voltage-dependent fluorophore/quencher pair. Whole-GUV patch-clamping allows ion transport and other voltage-dependent processes to be studied while controlling membrane composition, tension, and shape.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.