Evidence map›Paper›PMID 28003188›Full record

ArticleAmerican journal of physiology. Renal physiology2017

Insulin-like growth factor binding protein 7 and tissue inhibitor of metalloproteinases-2: differential expression and secretion in human kidney tubule cells.

David R Emlet, Nuria Pastor-Soler, Allison Marciszyn, Xiaoyan Wen, Hernando Gomez, William H Humphries, Seth Morrisroe, Jacob K Volpe, John A Kellum

Registry-linked trialOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Renal physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04530448 (Coronavirus Induced Acute Kidney Injury), which is not on this map. Cited by 77 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
77citing papers in PubMed, 2 pooled it
7.3field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT04530448 phase4terminatednot on this mapstarted 2020, after this paper: background citation

Coronavirus Induced Acute Kidney Injury: Prevention Using Urine Alkalinization

TypeinterventionalSponsorWest Virginia UniversityRan2020 to 2021Enrolled3ConditionsCOVID, Coronavirus, Coronavirus Infection, AKIArmsSodium bicarbonate, Standard of Care
3 · Its place in the literature

Who cites it

77 citing papers in PubMed, 2 syntheses or guidelines pooled it, 136 citations in OpenAlex.

  1. Pooled it
  2. Biomarkers of acute kidney injury in pediatric cardiac surgery.Pediatric nephrology (Berlin, Germany) · 2022
    Pooled it
  3. Trial
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  7. Article
  8. Article
  9. Review
  10. Review
  11. Article
  12. Article
  13. Article
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  16. Expression and distribution of MUC1 in the developing and adult kidney.American journal of physiology. Renal physiology · 2025
    Article
  17. Matrix metalloproteinases in kidney homeostasis and diseases: an update.American journal of physiology. Renal physiology · 2024
    Review
  18. Article
  19. Biomarkers in acute kidney injury.Annals of intensive care · 2024
    Review
  20. Observational

17 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

David R EmletCenter for Critical Care Nephrology, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Nuria Pastor-SolerRenal Electrolyte Division, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania; and.
Allison MarciszynRenal Electrolyte Division, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania; and.
Xiaoyan WenCenter for Critical Care Nephrology, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Hernando GomezCenter for Critical Care Nephrology, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
William H HumphriesB&B Microscopes, Limited, Pittsburgh, Pennsylvania.
Seth MorrisroeCenter for Critical Care Nephrology, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Jacob K VolpeCenter for Critical Care Nephrology, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
John A KellumCenter for Critical Care Nephrology, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania; kellum@pitt.edu.
University of Pittsburgh · US

Funding

Single Nephron and MetabolomicsP30DK079307 · NIDDK · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI KLEYMAN, THOMAS R · 2008 to 2022
$13.4M
NIDDK NIH HHS P30 DK079307
6 · The paper itself

Abstract

We have characterized the expression and secretion of the acute kidney injury (AKI) biomarkers insulin-like growth factor binding protein 7 (IGFBP7) and tissue inhibitor of metalloproteinases-2 (TIMP-2) in human kidney epithelial cells in primary cell culture and tissue. We established cell culture model systems of primary kidney cells of proximal and distal tubule origin and observed that both proteins are indeed expressed and secreted in both tubule cell types in vitro. However, TIMP-2 is both expressed and secreted preferentially by cells of distal tubule origin, while IGFBP7 is equally expressed across tubule cell types yet preferentially secreted by cells of proximal tubule origin. In human kidney tissue, strong staining of IGFBP7 was seen in the luminal brush-border region of a subset of proximal tubule cells, and TIMP-2 stained intracellularly in distal tubules. Additionally, while some tubular colocalization of both biomarkers was identified with the injury markers kidney injury molecule-1 and neutrophil gelatinase-associated lipocalin, both biomarkers could also be seen alone, suggesting the possibility for differential mechanistic and/or temporal profiles of regulation of these early AKI biomarkers from known markers of injury. Last, an in vitro model of ischemia-reperfusion demonstrated enhancement of secretion of both markers early after reperfusion. This work provides a rationale for further investigation of these markers for their potential role in the pathogenesis of acute kidney injury.

Indexed as

Acute Kidney InjuryBiomarkersHepatitis A Virus Cellular Receptor 1HumansInsulin-Like Growth Factor Binding ProteinsKidney Tubules, DistalKidney Tubules, ProximalLipocalin-2Organ SpecificityReperfusion InjuryTissue Inhibitor of Metalloproteinase-2BiomarkersHAVCR1 protein, humanHepatitis A Virus Cellular Receptor 1insulin-like growth factor binding protein-related protein 1Insulin-Like Growth Factor Binding ProteinsLipocalin-2TIMP2 protein, humanTissue Inhibitor of Metalloproteinase-2acute kidney injuryand TIMP-2biomarkersIGFBP7

Identifiers

PMID28003188
PMCPMC5336590
OpenAlexW2563393230

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.