ArticlePLoS pathogens2016
Cell-Based Screen Identifies Human Interferon-Stimulated Regulators of Listeria monocytogenes Infection.
Article in PLoS pathogens, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Human XIRP1 is a new podosome protein targeting cytosolic bacteria as part of the IFN-γ defense program.Journal of immunology (Baltimore, Md. : 1950) · 2026Article
- Gingipain proteases from the bacteriumProceedings of the National Academy of Sciences of the United States of America · 2026Article
- For Better or Worse: Type I Interferon Responses in Bacterial Infection.Pathogens (Basel, Switzerland) · 2025Review
- RIG-I drives protective type I interferon production by glial cells in response toFrontiers in immunology · 2025Article
- Emerging discoveries on the role of TRIM14: from diseases to immune regulation.Cell death discovery · 2024Review
- Interferon signaling drives epithelial metabolic reprogramming to promote secondary bacterial infection.PLoS pathogens · 2023Article
- Identification of KeyInternational journal of molecular sciences · 2023Article
- A genome-wide screen in macrophages identifies PTEN as required for myeloid restriction of Listeria monocytogenes infection.PLoS pathogens · 2023Article
- Human Listeriosis.Clinical microbiology reviews · 2023Review
- Inducible CRISPR activation screen for interferon-stimulated genes identifies OAS1 as a SARS-CoV-2 restriction factor.PLoS pathogens · 2022Article
- Overexpression screen of interferon-stimulated genes identifies RARRES3 as a restrictor ofeLife · 2021Article
- Toxoplasma gondii infection and its implications within the central nervous system.Nature reviews. Microbiology · 2021Review
- Article
- Whole transcriptome analysis of high and low IFN-α producers reveals differential response patterns following rhinovirus stimulation.Clinical & translational immunology · 2021Article
- UMP-CMP kinase 2 gene expression in macrophages is dependent on the IRF3-IFNAR signaling axis.PloS one · 2021Article
- TRIM14 Is a Key Regulator of the Type I IFN Response duringJournal of immunology (Baltimore, Md. : 1950) · 2020Article
- Oxysterols provide innate immunity to bacterial infection by mobilizing cell surface accessible cholesterol.Nature microbiology · 2020Article
- The ETS transcription factor ELF1 regulates a broadly antiviral program distinct from the type I interferon response.PLoS pathogens · 2019Article
- The interferon stimulated gene viperin, restricts Shigella. flexneri in vitro.Scientific reports · 2019Article
- A CRISPR screen identifies IFI6 as an ER-resident interferon effector that blocks flavivirus replication.Nature microbiology · 2018Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The type I interferon (IFN) activated transcriptional response is a critical antiviral defense mechanism, yet its role in bacterial pathogenesis remains less well characterized. Using an intracellular pathogen Listeria monocytogenes (Lm) as a model bacterial pathogen, we sought to identify the roles of individual interferon-stimulated genes (ISGs) in context of bacterial infection. Previously, IFN has been implicated in both restricting and promoting Lm growth and immune stimulatory functions in vivo. Here we adapted a gain-of-function flow cytometry based approach to screen a library of more than 350 human ISGs for inhibitors and enhancers of Lm infection. We identify 6 genes, including UNC93B1, MYD88, AQP9, and TRIM14 that potently inhibit Lm infection. These inhibitors act through both transcription-mediated (MYD88) and non-transcriptional mechanisms (TRIM14). Further, we identify and characterize the human high affinity immunoglobulin receptor FcγRIa as an enhancer of Lm internalization. Our results reveal that FcγRIa promotes Lm uptake in the absence of known host Lm internalization receptors (E-cadherin and c-Met) as well as bacterial surface internalins (InlA and InlB). Additionally, FcγRIa-mediated uptake occurs independently of Lm opsonization or canonical FcγRIa signaling. Finally, we established the contribution of FcγRIa to Lm infection in phagocytic cells, thus potentially linking the IFN response to a novel bacterial uptake pathway. Together, these studies provide an experimental and conceptual basis for deciphering the role of IFN in bacterial defense and virulence at single-gene resolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.