Evidence map›Paper›PMID 28000788›Full record

ArticleScientific reports2016

In vivo label-free photoacoustic flow cytography and on-the-spot laser killing of single circulating melanoma cells.

Yun He, Lidai Wang, Junhui Shi, Junjie Yao, Lei Li, Ruiying Zhang, Chih-Hsien Huang, Jun Zou, Lihong V Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
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  5. Review
  6. Article
  7. Review
  8. Article
  9. Review
  10. Article
  11. The sound of blood: photoacoustic imaging in blood analysis.Medicine in novel technology and devices · 2023
    Article
  12. Efficient label-freePhotoacoustics · 2023
    Article
  13. Article
  14. Near-infrared diffuse in vivo flow cytometry.Journal of biomedical optics · 2022
    Article
  15. Review
  16. Article
  17. Review
  18. Article
  19. Review
  20. Seeing through the Skin: Photoacoustic Tomography of Skin Vasculature and Beyond.JID innovations : skin science from molecules to population health · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yun HeOptical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
Lidai WangOptical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
Junhui ShiOptical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
Junjie YaoOptical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
Lei LiOptical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
Ruiying ZhangOptical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
Chih-Hsien HuangDepartment of Electrical and Computer Engineering, Texas A&M University, College Station, TX 77843, USA.
Jun ZouDepartment of Electrical and Computer Engineering, Texas A&M University, College Station, TX 77843, USA.
Lihong V WangOptical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.

Funding

PHOTON TUNNELING: SHEDDING NEW LIGHT ON BIOMEDICINEDP1EB016986 · NIBIB · WASHINGTON UNIVERSITY · PI WANG, LIHONG · 2012 to 2016
$3.8M
IN VIVO IMAGING OF SINGLE CIRCULATING CELLSR01CA186567 · NCI · WASHINGTON UNIVERSITY · PI WANG, LIHONG · 2013 to 2017
$3.6M
NCI NIH HHS R01 CA186567NIBIB NIH HHS DP1 EB016986
6 · The paper itself

Abstract

Metastasis causes as many as 90% of cancer-related deaths, especially for the deadliest skin cancer, melanoma. Since hematogenous dissemination of circulating tumor cells is the major route of metastasis, detection and destruction of circulating tumor cells are vital for impeding metastasis and improving patient prognosis. Exploiting the exquisite intrinsic optical absorption contrast of circulating melanoma cells, we developed dual-wavelength photoacoustic flow cytography coupled with a nanosecond-pulsed melanoma-specific laser therapy mechanism. We have successfully achieved in vivo label-free imaging of rare single circulating melanoma cells in both arteries and veins of mice. Further, the photoacoustic signal from a circulating melanoma cell immediately hardware-triggers a lethal pinpoint laser irradiation to kill it on the spot in a thermally confined manner without causing collateral damage. A pseudo-therapy study including both in vivo and in vitro experiments demonstrated the performance and the potential clinical value of our method, which can facilitate early treatment of metastasis by clearing circulating tumor cells from vasculature.

Indexed as

Neoplastic Cells, CirculatingAnimalsCell LineFemaleLasersLightMelanomaMiceMice, NudeNeoplasm MetastasisPhotoacoustic TechniquesSkin NeoplasmsSpectrum Analysis

Identifiers

PMID28000788
PMCPMC5175175

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.