ArticleScientific reports2016
Bioorthogonal chemical imaging of metabolic activities in live mammalian hippocampal tissues with stimulated Raman scattering.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 365 citations in OpenAlex.
- Pooled it
- Bioorthogonal Chemistry in Neurobiology: From Imaging to Proteomics.Chembiochem : a European journal of chemical biology · 2026Review
- Advanced multimodal imaging: FLIM, PLIM, and FluoRaman enabled by novel diarylacetylene probes.The Analyst · 2026Article
- Stimulated Raman Scattering Imaging Enabled Biomarker Discovery for Precision Medicine.Chemical & biomedical imaging · 2025Review
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- Uses of coherent Raman scattering microscopy in neuroscience.Frontiers in neuroscience · 2025Review
- Inspiring a convergent engineering approach to measure and model the tissue microenvironment.Heliyon · 2024Review
- Fast Real-Time Brain Tumor Detection Based on Stimulated Raman Histology and Self-Supervised Deep Learning Model.Journal of imaging informatics in medicine · 2024Article
- Biomedical applications, perspectives and tag design concepts in the cell - silent Raman window.RSC chemical biology · 2024Review
- Recent Advances in Enhancement of Raman Scattering Intensity for Biological Applications.Chemical & biomedical imaging · 2023Review
- Stretching the Bisalkyne Raman Spectral Palette Reveals a New Electrophilic Covalent Motif.Chemistry (Weinheim an der Bergstrasse, Germany) · 2023Article
- 3D Chemical Imaging by Fluorescence-detected Mid-Infrared Photothermal Fourier Light Field Microscopy.Chemical & biomedical imaging · 2023Article
- Raman Spectroscopy as a Tool to Study the Pathophysiology of Brain Diseases.International journal of molecular sciences · 2023Review
- Raman Spectroscopy for Chemical Biology Research.Journal of the American Chemical Society · 2022Review
- Towards Mapping Mouse Metabolic Tissue Atlas by Mid-Infrared Imaging with Heavy Water Labeling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Visualizing the lipid dynamics role in infrared neural stimulation using stimulated Raman scattering.Biophysical journal · 2022Article
- From Research to Diagnostic Application of Raman Spectroscopy in Neurosciences: Past and Perspectives.Free neuropathology · 2022Article
- From Raman to SESORRS: moving deeper into cancer detection and treatment monitoring.Chemical communications (Cambridge, England) · 2021Review
- A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems.RSC chemical biology · 2021Review
- Advances in stimulated Raman scattering imaging for tissues and animals.Quantitative imaging in medicine and surgery · 2021Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Brain is an immensely complex system displaying dynamic and heterogeneous metabolic activities. Visualizing cellular metabolism of nucleic acids, proteins, and lipids in brain with chemical specificity has been a long-standing challenge. Recent development in metabolic labeling of small biomolecules allows the study of these metabolisms at the global level. However, these techniques generally require nonphysiological sample preparation for either destructive mass spectrometry imaging or secondary labeling with relatively bulky fluorescent labels. In this study, we have demonstrated bioorthogonal chemical imaging of DNA, RNA, protein and lipid metabolism in live rat brain hippocampal tissues by coupling stimulated Raman scattering microscopy with integrated deuterium and alkyne labeling. Heterogeneous metabolic incorporations for different molecular species and neurogenesis with newly-incorporated DNA were observed in the dentate gyrus of hippocampus at the single cell level. We further applied this platform to study metabolic responses to traumatic brain injury in hippocampal slice cultures, and observed marked upregulation of protein and lipid metabolism particularly in the hilus region of the hippocampus within days of mechanical injury. Thus, our method paves the way for the study of complex metabolic profiles in live brain tissue under both physiological and pathological conditions with single-cell resolution and minimal perturbation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.