ArticleScientific reports2016
Unraveling the genetic basis of xylose consumption in engineered Saccharomyces cerevisiae strains.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 97 citations in OpenAlex.
- Sustainable Production of Biofuels from Lignocellulosic Biomass Using Microbial Applications: Status, Challenges and Prospects.Molecular biotechnology · 2026Review
- Positive selection of efficient ethanol producers from xylose at 45 °C in the yeast Ogataea polymorpha.Scientific reports · 2025Article
- Engineering Saccharomyces Cerevisiae With Novel Functional Xylose Isomerases From Rumen Microbiota for Enhanced Biofuel Production.Biotechnology journal · 2025Article
- Engineering a xylose fermenting yeast for lignocellulosic ethanol production.Nature chemical biology · 2025Article
- Engineering transcriptional regulatory networks for improving second-generation fuel ethanol production inSynthetic and systems biotechnology · 2025Review
- Construction of an economical xylose-utilizing Saccharomyces cerevisiae and its ethanol fermentation.FEMS yeast research · 2024Article
- Impact of xylose epimerase on sugar assimilation and sensing in recombinant Saccharomyces cerevisiae carrying different xylose-utilization pathways.Biotechnology for biofuels and bioproducts · 2023Article
- FromJournal of fungi (Basel, Switzerland) · 2023Review
- Integration of metabolism and regulation reveals rapid adaptability to growth on non-native substrates.Cell chemical biology · 2023Article
- Advances inJournal of fungi (Basel, Switzerland) · 2023Review
- PKA regulatory subunit Bcy1 couples growth, lipid metabolism, and fermentation during anaerobic xylose growth in Saccharomyces cerevisiae.PLoS genetics · 2023Article
- Review
- How adaptive laboratory evolution can boost yeast tolerance to lignocellulosic hydrolyses.Current genetics · 2022Review
- A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion.Biotechnology for biofuels and bioproducts · 2022Article
- Xylo-Oligosaccharide Utilization by EngineeredFrontiers in bioengineering and biotechnology · 2022Review
- D-Xylose Sensing inInternational journal of molecular sciences · 2021Review
- Exploring metal ion metabolisms to improve xylose fermentation in Saccharomyces cerevisiae.Microbial biotechnology · 2021Article
- Metabolic engineering considerations for the heterologous expression of xylose-catabolic pathways in Saccharomyces cerevisiae.PloS one · 2020Article
- Novel xylose transporter Cs4130 expands the sugar uptake repertoire in recombinantBiotechnology for biofuels · 2020Article
- Production of ethanol from Jerusalem artichoke by mycelial pellets.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
The development of biocatalysts capable of fermenting xylose, a five-carbon sugar abundant in lignocellulosic biomass, is a key step to achieve a viable production of second-generation ethanol. In this work, a robust industrial strain of Saccharomyces cerevisiae was modified by the addition of essential genes for pentose metabolism. Subsequently, taken through cycles of adaptive evolution with selection for optimal xylose utilization, strains could efficiently convert xylose to ethanol with a yield of about 0.46 g ethanol/g xylose. Though evolved independently, two strains carried shared mutations: amplification of the xylose isomerase gene and inactivation of ISU1, a gene encoding a scaffold protein involved in the assembly of iron-sulfur clusters. In addition, one of evolved strains carried a mutation in SSK2, a member of MAPKKK signaling pathway. In validation experiments, mutating ISU1 or SSK2 improved the ability to metabolize xylose of yeast cells without adaptive evolution, suggesting that these genes are key players in a regulatory network for xylose fermentation. Furthermore, addition of iron ion to the growth media improved xylose fermentation even by non-evolved cells. Our results provide promising new targets for metabolic engineering of C5-yeasts and point to iron as a potential new additive for improvement of second-generation ethanol production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.