ArticleScientific reports2016
The mechanism of a chitosan-collagen composite film used as biomaterial support for MC3T3-E1 cell differentiation.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 59 citations in OpenAlex.
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- A Hydrogel Scaffold Incorporating Fennel Seed Extract Induces Osteogenic Differentiation in Mesenchymal Stem Cells.Veterinary medicine and science · 2025Article
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- Combination of Polysaccharide Nanofibers Derived from Cellulose and Chitin Promotes the Adhesion, Migration and Proliferation of Mouse Fibroblast Cells.Nanomaterials (Basel, Switzerland) · 2022Article
- Carbon Dots-Mediated Fluorescent Scaffolds: Recent Trends in Image-Guided Tissue Engineering Applications.International journal of molecular sciences · 2021Review
- Up-regulation of TβRIII facilitates the osteogenesis of supraspinous ligament-derived fibroblasts from patients with ankylosing spondylitis.Journal of cellular and molecular medicine · 2021Article
- Characterization of Bacterial Cellulose-Based Wound Dressing in Different Order Impregnation of Chitosan and Collagen.Biomolecules · 2020Article
- Injectable osteogenic microtissues containing mesenchymal stromal cells conformally fill and repair critical-size defects.Biomaterials · 2019Article
- Interleukin-1 receptor antagonist protein (IL-1Ra) and miR-140 overexpression via pNNS-conjugated chitosan-mediated gene transfer enhances the repair of full-thickness cartilage defects in a rabbit model.Bone & joint research · 2019Article
- Specifications for Innovative, Enabling Biomaterials Based on the Principles of Biocompatibility Mechanisms.Frontiers in bioengineering and biotechnology · 2019Article
- Bone physiology as inspiration for tissue regenerative therapies.Biomaterials · 2018Review
- A proteomics study to explore the role of adsorbed serum proteins for PC12 cell adhesion and growth on chitosan and collagen/chitosan surfaces.Regenerative biomaterials · 2018Article
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- Biological Compatibility of a Polylactic Acid Composite Reinforced with Natural Chitosan Obtained from Shrimp Waste.Materials (Basel, Switzerland) · 2018Article
- Graphene Oxide Hybridized nHAC/PLGA Scaffolds Facilitate the Proliferation of MC3T3-E1 Cells.Nanoscale research letters · 2018Article
- The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect.Journal of Indian Society of PeriodontologyArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Natural composite biomaterials are good structural supports for bone cells to regenerate lost bone. Here, we report that a chitosan-collagen composite film accelerated osteoblast proliferation, differentiation and matrix mineralization in MC3T3-E1 cells. Intriguingly, we observed that the film enhanced the phosphorylation of Erk1/2. We showed that the chitosan-collagen composite film increased the transcriptional activity of Runx2, which is an important factor regulating osteoblast differentiation downstream of phosphorylated Erk1/2. Consistent with this observation, we found that the chitosan-collagen composite film increased the expression of osteoblastic marker genes, including Type I Collagen and Runx2 in MC3T3-E1 cells. We conclude that this film promoted osteoblast differentiation and matrix mineralization through an Erk1/2-activated Runx2 pathway. Our findings provide new evidence that chitosan-collagen composites are promising biomaterials for bone tissue engineering in bone defect-related diseases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.