ArticleScientific reports2016
Systematic Assessment of Seven Solvent and Solid-Phase Extraction Methods for Metabolomics Analysis of Human Plasma by LC-MS.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Plasma LC-HRMS metabolomics in parkinsonian syndromes: comparative evaluation of sample preparation protocols for exploratory metabolic signatures.Analytical and bioanalytical chemistry · 2026Article
- Metabolomic data of melittin-intervened murine cervical cancer cells based on liquid chromatography-mass spectrometry.Data in brief · 2026Article
- Sequential Penta-Omic Extraction Method Using Single Biospecimens of Post-mortem Human Brain.bioRxiv : the preprint server for biology · 2026Article
- Investigating Variability in Metabolomics: A Comparative Study of Analytical Platforms and Blood Matrices Using HPLC-HRMS.Molecules (Basel, Switzerland) · 2026Article
- Mass Spectrometry as a Tool for Metabolomics in Cancer.Advances in experimental medicine and biology · 2026Review
- LC-MS-Based Metabolomics and Proteomics Sample Preparation of Urine: Methods and Multi-Omics Integration.Advances in experimental medicine and biology · 2026Review
- Multiclass Assays for Measuring Environmental Chemical Mixture Exposure: Analytical Methodologies and Applications in Exposomics Research.Metabolites · 2025Review
- Evaluating Novel Direct Injection Liquid Chromatography-Mass Spectrometry Method and Extraction-Based Workflows for Untargeted Lipidomics of Extracellular Vesicles.Journal of proteome research · 2025Article
- A Taguchi Design of Experiments Approach for Untargeted Metabolomics Sample Preparation Optimization.Journal of biomolecular techniques : JBT · 2025Article
- Nanoparticle-assisted strategies in mass spectrometry-based metabolite analysis: recent advances across analytical workflow.Mikrochimica acta · 2025Review
- Impact of myocardial revascularization surgery on the plasma metabolome.Metabolomics : Official journal of the Metabolomic Society · 2025Article
- Mass Spectral Data of Primary and Secondary Metabolites Changes in Medicinal Plants by Solvent Polarity.Scientific data · 2025Article
- Development of plant extracts as substrates for untargeted transporter substrate identification inFrontiers in plant science · 2025Article
- Evaluating protocols for reproducible targeted metabolomics by NMR.The Analyst · 2024Article
- Memory effects of prior subculture may impact the quality of multiomic perturbation profiles.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Phospholipid Removal for Enhanced Chemical Exposomics in Human Plasma.Environmental science & technology · 2023Article
- Comparison between 5 extractions methods in either plasma or serum to determine the optimal extraction and matrix combination for human metabolomics.Cellular & molecular biology letters · 2023Article
- Comparison of the Determination of Some Antihypertensive Drugs in Clinical Human Plasma Samples by Solvent Front Position Extraction and Precipitation Modes.Molecules (Basel, Switzerland) · 2023Article
- Rapid Multi-Omics Sample Preparation for Mass Spectrometry.Analytical chemistry · 2023Article
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3 authors.
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Abstract
The comparison of extraction methods for global metabolomics is usually executed in biofluids only and focuses on metabolite coverage and method repeatability. This limits our detailed understanding of extraction parameters such as recovery and matrix effects and prevents side-by-side comparison of different sample preparation strategies. To address this gap in knowledge, seven solvent-based and solid-phase extraction methods were systematically evaluated using standard analytes spiked into both buffer and human plasma. We compared recovery, coverage, repeatability, matrix effects, selectivity and orthogonality of all methods tested for non-lipid metabolome in combination with reversed-phased and mixed-mode liquid chromatography mass spectrometry analysis (LC-MS). Our results confirmed wide selectivity and excellent precision of solvent precipitations, but revealed their high susceptibility to matrix effects. The use of all seven methods showed high overlap and redundancy which resulted in metabolite coverage increases of 34-80% depending on LC-MS method employed as compared to the best single extraction protocol (methanol/ethanol precipitation) despite 7x increase in MS analysis time and sample consumption. The most orthogonal methods to methanol-based precipitation were ion-exchange solid-phase extraction and liquid-liquid extraction using methyl-tertbutyl ether. Our results help facilitate rational design and selection of sample preparation methods and internal standards for global metabolomics.
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