ReviewApplied microbiology and biotechnology2017
Tunable recombinant protein expression in E. coli: promoter systems and genetic constraints.
Review in Applied microbiology and biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- A cumate-inducible promoter system for tuned gene expression and magnetosome overproduction in magnetotactic bacteria.Applied microbiology and biotechnology · 2026Article
- Engineering smart bacteria for the next generation.Cell reports. Medicine · 2026Review
- A modular biodevice assembly tool kit for combinatorial screening and benchmarking multiple cell surface display platforms in Escherichia coli.Journal of biological engineering · 2026Article
- Integration of pathway balance and protein fusion enables de novo biosynthesis of (+)-bicyclogermacrene in Escherichia coli.Bioresources and bioprocessing · 2026Article
- A single vector system for tunable and homogeneous dual gene expression in Escherichia coli.Scientific reports · 2025Article
- Delaying production with prokaryotic inducible expression systems.Microbial cell factories · 2024Review
- A genetic toolbox to empower Paracoccus pantotrophus DSM 2944 as a metabolically versatile SynBio chassis.Microbial cell factories · 2024Article
- OptimizingFrontiers in microbiology · 2024Review
- Expanded MutaT7 toolkit efficiently and simultaneously accesses all possible transition mutations in bacteria.Nucleic acids research · 2023Article
- Venom biotechnology: casting light on nature's deadliest weapons using synthetic biology.Frontiers in bioengineering and biotechnology · 2023Review
- Standardization of regulatory nodes for engineering heterologous gene expression: a feasibility study.Microbial biotechnology · 2022Article
- Process intensification at the expression system level for the production of 1-phosphate aldolase in antibiotic-free E. coli fed-batch cultures.Journal of industrial microbiology & biotechnology · 2022Article
- Implementing Multi-Enzyme Biocatalytic Systems Using Nanoparticle Scaffolds.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Substrate-inducible and antibiotic-free high-level 4-hydroxyvaleric acid production in engineeredFrontiers in bioengineering and biotechnology · 2022Article
- Engineering AraC to make it responsive to light instead of arabinose.Nature chemical biology · 2021Article
- Impact of the Expression System on Recombinant Protein Production inFrontiers in microbiology · 2021Article
- Restoration of wild-type motility to flagellin-knockoutCurrent research in biotechnology · 2020Article
- Evolution ofInternational journal of molecular sciences · 2020Review
- Strategies for enhancing gene expression in Escherichia coli.Applied microbiology and biotechnology · 2020Review
- Escherichia coli σMicrobial cell factories · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tuning of transcription is a promising strategy to overcome challenges associated with a non-suitable expression rate like outgrowth of segregants, inclusion body formation, metabolic burden and inefficient translocation. By adjusting the expression rate-even on line-to purposeful levels higher product titres and more cost-efficient production processes can be achieved by enabling culture long-term stability and constant product quality. Some tunable systems are registered for patents or already commercially available. Within this contribution, we discuss the induction mechanisms of various Escherichia coli inherent promoter systems with respect to their tunability and review studies using these systems for expression tuning. According to the current level of knowledge, some promoter systems were successfully used for expression tuning, and in some cases, analytical evidence on single-cell level is still pending. However, only a few studies using tunable strains apply a suitable process control strategy. So far, expression tuning has only gathered little attention, but we anticipate that expression tuning harbours great potential for enabling and optimizing the production of a broad spectrum of products in E. coli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.