Evidence map›Paper›PMID 27999668›Full record

ArticleJournal of animal science and biotechnology2016

Development of a vivo rabbit ligated intestinal Loop Model for HCMV infection.

Jin Tang, Qiaoxing Wu, Xinming Tang, Ruihan Shi, Jingxia Suo, Guangping Huang, Junqing An, Jingyuan Wang, Jinling Yang, Wenzhuo Hao and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of animal science and biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 86% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Jin TangNational animal protozoa laboratory,College of VeterinaryMedicine, China Agricultural University, Beijing, 100193 China.
Qiaoxing WuLaboratory of Animal Pathology & Public Health, College of Veterinary Medicine, China Agricultural University, Beijing, 100193 China.
Xinming TangNational animal protozoa laboratory,College of VeterinaryMedicine, China Agricultural University, Beijing, 100193 China.
Ruihan ShiLaboratory of Animal Pathology & Public Health, College of Veterinary Medicine, China Agricultural University, Beijing, 100193 China.
Jingxia SuoNational animal protozoa laboratory,College of VeterinaryMedicine, China Agricultural University, Beijing, 100193 China.
Guangping HuangNational animal protozoa laboratory,College of VeterinaryMedicine, China Agricultural University, Beijing, 100193 China.
Junqing AnLaboratory of Animal Pathology & Public Health, College of Veterinary Medicine, China Agricultural University, Beijing, 100193 China.
Jingyuan WangLaboratory of Animal Pathology & Public Health, College of Veterinary Medicine, China Agricultural University, Beijing, 100193 China.
Jinling YangLaboratory of Animal Pathology & Public Health, College of Veterinary Medicine, China Agricultural University, Beijing, 100193 China.
Wenzhuo HaoLaboratory of Animal Pathology & Public Health, College of Veterinary Medicine, China Agricultural University, Beijing, 100193 China.
Ruiping SheLaboratory of Animal Pathology & Public Health, College of Veterinary Medicine, China Agricultural University, Beijing, 100193 China.
Xun SuoNational animal protozoa laboratory,College of VeterinaryMedicine, China Agricultural University, Beijing, 100193 China.
China Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHuman Cytomegalovirus (HCMV) infections can be found throughout the body, especially in epithelial tissue. Animal model was established by inoculation of HCMV (strain AD-169) or coinoculation with Hepatitis E virus (HEV) into the ligated sacculus rotundus and vermiform appendix in living rabbits. The specimens were collected from animals sacrificed 1 and a half hours after infection.

resultsThe virus was found to be capable of reproducing in these specimens through RT-PCR and Western-blot. Severe inflammation damage was found in HCMV-infected tissue. The viral protein could be detected in high amounts in the mucosal epithelium and lamina propria by immunohistochemistry and immunofluorescense. Moreover, there are strong positive signals in lymphocytes, macrophages, and lymphoid follicles. Quantitative statistics indicate that lymphocytes among epithlium cells increased significantly in viral infection groups.

conclusionsThe results showed that HCMV or HEV + HCMV can efficiently infect in rabbits by vivo ligated intestine loop inoculation. The present study successfully developed an infective model

Indexed as

HCMVHEVImmunohistochemistry and confocal immunofluorescenceInoculating ligated intestine in vivoPathological lesionRabbit sacculus rotundusVermiform appendix

Identifiers

PMID27999668
PMCPMC5154130
OpenAlexW2564136975

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.