Evidence map›Paper›PMID 27999395›Full record

ArticleGenes2016

Stem-Loop RT-qPCR as an Efficient Tool for the Detection and Quantification of Small RNAs in Giardia lamblia.

Jaime Marcial-Quino, Saúl Gómez-Manzo, Francisco Fierro, America Vanoye-Carlo, Yadira Rufino-González, Edgar Sierra-Palacios, Adriana Castillo-Villanueva, Rosa Angélica Castillo-Rodríguez, Eduardo Rodríguez-Bustamante, Roberto Arreguin-Espinosa and 1 more

Open access · goldAbstract read
In one paragraph

Article in Genes, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.3field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
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  3. Review
  4. Article
  5. Epidermal cell-patterning genes of the stem parasitic plantPlant biotechnology (Tokyo, Japan) · 2021
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

Jaime Marcial-QuinoCONACYT-Instituto Nacional de Pediatría, Secretaría de Salud, Ciudad de México 04530, Mexico. jmarcialqu@conacyt.mx.
Saúl Gómez-ManzoLaboratorio de Bioquímica Genética, Instituto Nacional de Pediatría, Secretaría de Salud, Ciudad de México 04530, Mexico. saulmanzo@ciencias.unam.mx.
Francisco FierroLaboratorio de Ingeniería Genética y Metabolitos Secundarios, Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Ciudad de México 09340, Mexico. degfff@yahoo.com.
America Vanoye-CarloLaboratorio de Neurociencias, Instituto Nacional de Pediatría, Secretaría de Salud, Ciudad de México 04530, Mexico. america_vc@yahoo.com.mx.
Yadira Rufino-GonzálezLaboratorio de Parasitología Experimental, Instituto Nacional de Pediatría, Secretaría de Salud, Ciudad de México 04530, Mexico. yadirg@gmail.com.
Edgar Sierra-PalaciosColegio de Ciencias y Humanidades, Plantel Casa Libertad, Universidad Autónoma de la Ciudad de México, Ciudad de México 09620, Mexico. edgar.sierra@uacm.edu.mx.
Adriana Castillo-VillanuevaLaboratorio de Bioquímica Genética, Instituto Nacional de Pediatría, Secretaría de Salud, Ciudad de México 04530, Mexico. acastilloinp@gmail.com.
Rosa Angélica Castillo-RodríguezCONACYT-Instituto Nacional de Pediatría, Secretaría de Salud, Ciudad de México 04530, Mexico. racastilloro@conacyt.mx.
Eduardo Rodríguez-BustamanteDepartamento de Química de Biomacromoléculas, Instituto de Química, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad Universitaria, Ciudad de México 04510, Mexico. e-rodriguez-bustamante@ciencias.unam.mx.
Roberto Arreguin-EspinosaDepartamento de Química de Biomacromoléculas, Instituto de Química, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad Universitaria, Ciudad de México 04510, Mexico. arrespin@unam.mx.
Horacio Reyes-VivasLaboratorio de Bioquímica Genética, Instituto Nacional de Pediatría, Secretaría de Salud, Ciudad de México 04530, Mexico. hreyesvivas@yahoo.com.mx.
Secretaria de Salud · MXUniversidad Nacional Autónoma de México · MXUniversidad Autónoma de la Ciudad de México · MXUniversidad Autónoma Metropolitana · MX

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Stem-loop quantitative reverse transcription PCR (RT-qPCR) is a molecular technique used for identification and quantification of individual small RNAs in cells. In this work, we used a Universal ProbeLibrary (UPL)-based design to detect-in a rapid, sensitive, specific, and reproducible way-the small nucleolar RNA (snoRNA) GlsR17 and its derived miRNA (miR2) of

Indexed as

Giardia lambliamicroRNAsmall nucleolar RNAstem-loop RT-qPCR

Identifiers

PMID27999395
PMCPMC5192507
OpenAlexW2565259330

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.