Evidence map›Paper›PMID 27999291›Full record

ArticleSensors (Basel, Switzerland)2016

Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen.

Dong Seok Kim, Yong Tae Kim, Seok Bok Hong, Jinwoon Kim, Nam Su Huh, Moon-Keun Lee, Seok Jae Lee, Byeong Il Kim, In Soo Kim, Yun Suk Huh and 1 more

Abstract read
In one paragraph

Article in Sensors (Basel, Switzerland), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Dong Seok KimDepartment of Chemical Engineering, Kangwon National University, Samcheok 25913, Korea. nyongtory91@gmail.com.
Yong Tae KimNanobio Application Team, National NanoFab Center (NNFC), Daejeon 34141, Korea. ytkim@nnfc.re.kr.
Seok Bok HongDepartment of Chemical Engineering, Kangwon National University, Samcheok 25913, Korea. skychojja@gmail.com.
Jinwoon KimDepartment of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, Korea. anlfkrn2000@gmail.com.
Nam Su HuhDepartment of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, Korea. bioheo6@gmail.com.
Moon-Keun LeeNanobio Application Team, National NanoFab Center (NNFC), Daejeon 34141, Korea. mklee@nnfc.re.kr.
Seok Jae LeeNanobio Application Team, National NanoFab Center (NNFC), Daejeon 34141, Korea. sjlee@nnfc.re.kr.
Byeong Il KimResearch and Development Center, Tomorrow & Solution (T&S), E19-604, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Korea. kbiset@gmail.com.
In Soo KimResearch Center 2, Eudipia Inc., Cheongju 361-951, Korea. i.s.kim@eudipia.com.
Yun Suk HuhDepartment of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, Korea. yunsuk.huh@inha.ac.kr.
Bong Gill ChoiDepartment of Chemical Engineering, Kangwon National University, Samcheok 25913, Korea. bgchoi@kangwon.ac.kr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, we developed lateral flow assay (LFA) biosensors for the detection of hepatitis B surface antigens using well-controlled gold nanoparticles (AuNPs). To enhance colorimetric signals, a seeded growth method was used for the preparation of size-controlled AuNPs with a narrow size distribution. Different sizes of AuNPs in the range of 342-137.8 nm were conjugated with antibodies and then optimized for the efficient detection of LFA biosensors. The conjugation stability was investigated by UV-vis spectroscopy of AuNP dispersion at various pH values and concentrations of antibody. Based on optimized conjugation conditions, the use of 42.7 ± 0.8 nm AuNPs exhibited superior performance for the detection of LFAs relative to other sizes of AuNPs.

Indexed as

Particle SizeColorGoldHepatitis B Surface AntigensHumansMetal NanoparticlesReagent Kits, DiagnosticRheologySpectrophotometry, UltravioletGoldHepatitis B Surface AntigensReagent Kits, Diagnosticconjugationgoldhepatitis B surface antigenlateral flow assaynanoparticle

Identifiers

PMID27999291
PMCPMC5191134

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.