ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Production of recombinant human epidermal growth factor in Pichia pastoris.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 2 of them syntheses that pooled it.
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Who cites it
18 citing papers in PubMed, 2 syntheses or guidelines pooled it, 60 citations in OpenAlex.
- Efficacy of Cosmetic Debridement and Suture With Recombinant Human EGF in Maxillofacial Trauma: A Meta-Analysis.Journal of cosmetic dermatology · 2025Pooled it
- A Systematic Review of the Potential of Pichia pastoris (Komagataella phaffii) as an Alternative Host for Biologics Production.Molecular biotechnology · 2024Pooled it
- Efficient production of L-asparaginase using recombinant Pichia pastoris and strategy to eliminate glutaminase activity.Biotechnology letters · 2026Article
- Molecular engineering of Komagataella phaffii for venom toxin production.Applied microbiology and biotechnology · 2026Review
- Beyond Purification: Evolving Roles of Fusion Tags in Biotechnology.Current issues in molecular biology · 2025Review
- Impact of cell wall polysaccharide modifications on the performance of Pichia pastoris: novel mutants with enhanced fitness and functionality for bioproduction applications.Microbial cell factories · 2024Article
- Alternative PCR-Based Approaches for Generation ofMicroorganisms · 2023Article
- EGF, a veteran of wound healing: highlights on its mode of action, clinical applications with focus on wound treatment, and recent drug delivery strategies.Archives of pharmacal research · 2023Review
- Economic optimization of expression of soluble human epidermal growth factor in Escherichia coli.Biotechnology letters · 2022Article
- Article
- A toolkit for recombinant production of seven human EGF family growth factors in active conformation.Scientific reports · 2022Article
- Current advances ofFrontiers in microbiology · 2022Review
- Improved Production of Recombinant Myrosinase inInternational journal of molecular sciences · 2021Article
- An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris).BMC biotechnology · 2020Article
- Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins.Journal of cellular physiology · 2020Review
- Optimization of Secreted Recombinant Human Epidermal Growth Factor Production Using Pectate Lyase B fromJournal of pharmacy & bioallied sciences · 2019Article
- Improved strategy for isoleucineJournal of biomolecular NMR · 2019Article
- High Efficient Expression and Purification of Human Epidermal Growth Factor inInternational journal of molecular sciences · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study was carried out to express human epidermal growth factor (hEGF) in Pichia pastoris GS115. For this aim, the hEGF gene was cloned into the pPIC9K expression vector, and then integrated into P. pastoris by electroporation. ELISA-based assay showed that the amount of hEGF secreted into the medium can be affected by the fermentation conditions especially by culture medium, pH and temperature. The best medium for the optimal hEGF production was BMMY buffered at a pH range of 6.0 and 7.0. The highest amount of hEGF with an average yield of 2.27μg/mL was obtained through an induction of the culture with 0.5% (v/v) methanol for 60h. The artificial neural network (ANN) analysis revealed that changes in both pH and temperature significantly affected the hEGF production with the pH change had slightly higher impact on hEGF production than variations in the temperature.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.