ArticlePLoS genetics2016
Cell Cycle Constraints and Environmental Control of Local DNA Hypomethylation in α-Proteobacteria.
Article in PLoS genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
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- Intercellular mosaic methylation in fast-growingNAR molecular medicine · 2024Article
- Comparison of CcrM-dependent methylation inJournal of bacteriology · 2024Article
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- Comparison of CcrM-dependent methylation inbioRxiv : the preprint server for biology · 2024Article
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- AamA-mediated epigenetic control of genome-wide gene expression and phenotypic traits inMicrobial genomics · 2023Article
- Marine DNA methylation patterns are associated with microbial community composition and inform virus-host dynamics.Microbiome · 2022Article
- The Mycobacterial DNA Methyltransferase HsdM Decreases Intrinsic Isoniazid Susceptibility.Antibiotics (Basel, Switzerland) · 2021Article
- A rationally designed oral vaccine induces immunoglobulin A in the murine gut that directs the evolution of attenuated Salmonella variants.Nature microbiology · 2021Article
- Transcriptional rewiring of the GcrA/CcrM bacterial epigenetic regulatory system in closely related bacteria.PLoS genetics · 2021Article
- Contribution of DNA adenine methylation to gene expression heterogeneity in Salmonella enterica.Nucleic acids research · 2020Article
- Article
- Epigenomic characterization of Clostridioides difficile finds a conserved DNA methyltransferase that mediates sporulation and pathogenesis.Nature microbiology · 2020Article
- The bacterial epigenome.Nature reviews. Microbiology · 2020Review
- Bacterial cell cycle and growth phase switch by the essential transcriptional regulator CtrA.Nucleic acids research · 2019Article
- The complete methylome of an entomopathogenic bacterium reveals the existence of loci with unmethylated Adenines.Scientific reports · 2018Article
- Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heritable DNA methylation imprints are ubiquitous and underlie genetic variability from bacteria to humans. In microbial genomes, DNA methylation has been implicated in gene transcription, DNA replication and repair, nucleoid segregation, transposition and virulence of pathogenic strains. Despite the importance of local (hypo)methylation at specific loci, how and when these patterns are established during the cell cycle remains poorly characterized. Taking advantage of the small genomes and the synchronizability of α-proteobacteria, we discovered that conserved determinants of the cell cycle transcriptional circuitry establish specific hypomethylation patterns in the cell cycle model system Caulobacter crescentus. We used genome-wide methyl-N6-adenine (m6A-) analyses by restriction-enzyme-cleavage sequencing (REC-Seq) and single-molecule real-time (SMRT) sequencing to show that MucR, a transcriptional regulator that represses virulence and cell cycle genes in S-phase but no longer in G1-phase, occludes 5'-GANTC-3' sequence motifs that are methylated by the DNA adenine methyltransferase CcrM. Constitutive expression of CcrM or heterologous methylases in at least two different α-proteobacteria homogenizes m6A patterns even when MucR is present and affects promoter activity. Environmental stress (phosphate limitation) can override and reconfigure local hypomethylation patterns imposed by the cell cycle circuitry that dictate when and where local hypomethylation is instated.
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