Evidence map›Paper›PMID 27997133›Full record

ArticleBiomacromolecules2017

Injectable, Guest-Host Assembled Polyethylenimine Hydrogel for siRNA Delivery.

Leo L Wang, Janna N Sloand, Ann C Gaffey, Chantel M Venkataraman, Zhichun Wang, Alen Trubelja, Daniel A Hammer, Pavan Atluri, Jason A Burdick

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomacromolecules, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

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  14. Theranostic small interfering RNA nanoparticles in cancer precision nanomedicine.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2020
    Review
  15. Article
  16. Article
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  19. Advances in Biomaterials for Drug Delivery.Advanced materials (Deerfield Beach, Fla.) · 2018
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Leo L WangDepartment of Bioengineering, ‡Division of Cardiovascular Surgery, Department of Surgery, and §Department of Chemical and Biomolecular Engineering, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States.
Janna N SloandDepartment of Bioengineering, ‡Division of Cardiovascular Surgery, Department of Surgery, and §Department of Chemical and Biomolecular Engineering, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States.
Ann C GaffeyDepartment of Bioengineering, ‡Division of Cardiovascular Surgery, Department of Surgery, and §Department of Chemical and Biomolecular Engineering, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States.
Chantel M VenkataramanDepartment of Bioengineering, ‡Division of Cardiovascular Surgery, Department of Surgery, and §Department of Chemical and Biomolecular Engineering, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States.
Zhichun WangDepartment of Bioengineering, ‡Division of Cardiovascular Surgery, Department of Surgery, and §Department of Chemical and Biomolecular Engineering, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States.
Alen TrubeljaDepartment of Bioengineering, ‡Division of Cardiovascular Surgery, Department of Surgery, and §Department of Chemical and Biomolecular Engineering, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States.
Daniel A HammerDepartment of Bioengineering, ‡Division of Cardiovascular Surgery, Department of Surgery, and §Department of Chemical and Biomolecular Engineering, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States.
Pavan AtluriDepartment of Bioengineering, ‡Division of Cardiovascular Surgery, Department of Surgery, and §Department of Chemical and Biomolecular Engineering, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States.
Jason A BurdickDepartment of Bioengineering, ‡Division of Cardiovascular Surgery, Department of Surgery, and §Department of Chemical and Biomolecular Engineering, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States.ORCID 0000-0002-2006-332X

Funding

Injectable Hydrogels for miR302 Mimic Delivery After Myocardial InfarctionF30HL134255 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI WANG, LEO LE · 2016 to 2018
$111k
NHLBI NIH HHS F30 HL134255
6 · The paper itself

Abstract

While siRNA has tremendous potential for therapeutic applications, advancement is limited by poor delivery systems. Systemically, siRNAs are rapidly degraded, may have off-target silencing, and necessitate high working concentrations. To overcome this, we developed an injectable, guest-host assembled hydrogel between polyethylenimine (PEI) and polyethylene glycol (PEG) for local siRNA delivery. Guest-host modified polymers assembled with siRNAs to form polyplexes that had improved transfection and viability compared to PEI. At higher concentrations, these polymers assembled into shear-thinning hydrogels that rapidly self-healed. With siRNA encapsulation, the assemblies eroded as polyplexes which were active and transfected cells, observed by Cy3-siRNA uptake or GFP silencing in vitro. When injected into rat myocardium, the hydrogels localized polyplex release, observed by uptake of Cy5.5-siRNA and silencing of GFP for 1 week in a GFP-expressing rat. These results illustrate the potential for this system to be applied for therapeutic siRNA delivery, such as in cardiac pathologies.

Indexed as

Drug Delivery SystemsGene SilencingAnimalsGene Transfer TechniquesGreen Fluorescent ProteinsHydrogel, Polyethylene Glycol DimethacrylateMaleMyocardiumPolyethyleneiminePolymersRatsRats, WistarRNA, Small InterferingGreen Fluorescent ProteinsHydrogel, Polyethylene Glycol DimethacrylatePolyethyleneiminePolymersRNA, Small Interfering

Identifiers

PMID27997133
PMCPMC10953697

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.