ArticleScientific reports2016
In vitro synthesis of a Major Facilitator Transporter for specific active transport across Droplet Interface Bilayers.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Cell-Free Production Systems in Droplet Microfluidics.Advances in biochemical engineering/biotechnology · 2023Review
- Challenges and opportunities in achieving the full potential of droplet interface bilayers.Nature chemistry · 2022Review
- How lipids affect the energetics of co-translational alpha helical membrane protein folding.Biochemical Society transactions · 2022Review
- Chemical Communication in Artificial Cells: Basic Concepts, Design and Challenges.Frontiers in molecular biosciences · 2022Review
- Cell-Free Synthesis Strategies to Probe Co-translational Folding of Proteins Within Lipid Membranes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Cell-Free Expression to Probe Co-Translational Insertion of an Alpha Helical Membrane Protein.Frontiers in molecular biosciences · 2022Article
- The membrane transporter lactose permease increases lipid bilayer bending rigidity.Biophysical journal · 2021Article
- Cotranslational recruitment of ribosomes in protocells recreates a translocon-independent mechanism of proteorhodopsin biogenesis.iScience · 2021Article
- Integrating Membrane Transporter Proteins into Droplet Interface Bilayers.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Membrane protein mediated bilayer communication in networks of droplet interface bilayers.Communications chemistry · 2020Article
- Cell-free expression tools to study co-translational folding of alpha helical membrane transporters.Scientific reports · 2020Article
- Cell-Free Protein Synthesis: A Promising Option for Future Drug Development.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2020Review
- Synthetic tissues.Emerging topics in life sciences · 2019Article
- Co-Translational Protein Folding in Lipid Membranes.Trends in biochemical sciences · 2019Review
- Cell-free microcompartmentalised transcription-translation for the prototyping of synthetic communication networks.Current opinion in biotechnology · 2019Review
- Probing membrane protein properties using droplet interface bilayers.Experimental biology and medicine (Maywood, N.J.) · 2019Article
- Direct protein-lipid interactions shape the conformational landscape of secondary transporters.Nature communications · 2018Article
- Structure formation during translocon-unassisted co-translational membrane protein folding.Scientific reports · 2017Article
- Cell-free synthetic biology forBiochemical Society transactions · 2017Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Nature encapsulates reactions within membrane-bound compartments, affording sequential and spatial control over biochemical reactions. Droplet Interface Bilayers are evolving into a valuable platform to mimic this key biological feature in artificial systems. A major issue is manipulating flow across synthetic bilayers. Droplet Interface Bilayers must be functionalised, with seminal work using membrane-inserting toxins, ion channels and pumps illustrating the potential. Specific transport of biomolecules, and notably transport against a concentration gradient, across these bilayers has yet to be demonstrated. Here, we successfully incorporate the archetypal Major Facilitator Superfamily transporter, lactose permease, into Droplet Interface Bilayers and demonstrate both passive and active, uphill transport. This paves the way for controllable transport of sugars, metabolites and other essential biomolecular substrates of this ubiquitous transporter superfamily in DIB networks. Furthermore, cell-free synthesis of lactose permease during DIB formation also results in active transport across the interface bilayer. This adds a specific disaccharide transporter to the small list of integral membrane proteins that can be synthesised via in vitro transcription/translation for applications of DIB-based artificial cell systems. The introduction of a means to promote specific transport of molecules across Droplet Interface Bilayers against a concentration gradient gives a new facet to droplet networks.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.