ArticlePlant cell reports2017
Efficient CRISPR/Cas9-based gene knockout in watermelon.
Article in Plant cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
101 citing papers in PubMed, 1 synthesis or guideline pooled it, 262 citations in OpenAlex.
- Genome editing for vegetable crop improvement: Challenges and future prospects.Frontiers in genetics · 2022Pooled it
- Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026Review
- Precision editing to improve fruit traits: CRISPR/Cas into the picture.Protoplasma · 2026Review
- Genomic resources and genetic improvement of vital tropical and subtropical fruit crops: current status and prospects.AoB PLANTS · 2025Review
- Map-based cloning and functional validation of ClChlH governing yellow rind color in watermelon (Citrullus lanatus).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Genome editing in vegetable crops: a new era of sustainable agriculture.Molecular biology reports · 2025Review
- CRISPR/Cas9-mediated editing of carotenoid biosynthesis genes alters carotenoid concentrations in kiwifruit.BMC plant biology · 2025Article
- Multi-omics analyses unveil dual genetic loci governing four distinct watermelon flesh color phenotypes.Molecular horticulture · 2025Article
- Harnessing promoter elements to enhance gene editing in plants: perspectives and advances.Plant biotechnology journal · 2025Review
- A comprehensive all-in-one CRISPR toolbox for large-scale screens in plants.The Plant cell · 2025Article
- Novel methods for genetic transformation of watermelon (Molecular breeding : new strategies in plant improvement · 2025Article
- Fresh-cut watermelon: postharvest physiology, technology, and opportunities for quality improvement.Frontiers in genetics · 2025Review
- Unlocking the potential of 'Egusi' melon (Frontiers in plant science · 2025Review
- A natural variant of NON-RIPENING promotes fruit ripening in watermelon.The Plant cell · 2024Article
- Article
- EditingInternational journal of molecular sciences · 2024Article
- A review on strategies for crop improvement against drought stress through molecular insights.3 Biotech · 2024Review
- Parthenocarpy inPlants (Basel, Switzerland) · 2023Review
- Development of an Agrobacterium-delivered codon-optimized CRISPR/Cas9 system for chickpea genome editing.Protoplasma · 2023Article
- Developing a highly efficient CGBE base editor in watermelon.Horticulture research · 2023Article
41 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageCRISPR/Cas9 system can precisely edit genomic sequence and effectively create knockout mutations in T0 generation watermelon plants. Genome editing offers great advantage to reveal gene function and generate agronomically important mutations to crops. Recently, RNA-guided genome editing system using the type II clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) has been applied to several plant species, achieving successful targeted mutagenesis. Here, we report the genome of watermelon, an important fruit crop, can also be precisely edited by CRISPR/Cas9 system. ClPDS, phytoene desaturase in watermelon, was selected as the target gene because its mutant bears evident albino phenotype. CRISPR/Cas9 system performed genome editing, such as insertions or deletions at the expected position, in transfected watermelon protoplast cells. More importantly, all transgenic watermelon plants harbored ClPDS mutations and showed clear or mosaic albino phenotype, indicating that CRISPR/Cas9 system has technically 100% of genome editing efficiency in transgenic watermelon lines. Furthermore, there were very likely no off-target mutations, indicated by examining regions that were highly homologous to sgRNA sequences. Our results show that CRISPR/Cas9 system is a powerful tool to effectively create knockout mutations in watermelon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.