ArticleSpringerPlus2016
Simultaneous detection of influenza A subtypes of H3N2 virus, pandemic (H1N1) 2009 virus and reassortant avian H7N9 virus in humans by multiplex one-step real-time RT-PCR assay.
Article in SpringerPlus, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 27 citations in OpenAlex.
- Plasma nontargeted metabolomics study of H1N1 and H3N2 influenza in children.Frontiers in cellular and infection microbiology · 2025Article
- Profiling lipid mediators in serum from children with H1N1 influenza.Scientific reports · 2024Article
- Comparison between five PCR techniques for the diagnosis of SARS-CoV-2.Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia · 2022Article
- Reverse transcription recombinase-aided amplification assay for H5 subtype avian influenza virus.Virology journal · 2022Article
- A changing trend in diagnostic methods of Influenza A (H3N2) virus in human: a review.3 Biotech · 2021Review
- Comparison and clinical validation of qPCR assays targeting Leishmania 18S rDNA and HSP70 genes in patients with American Tegumentary Leishmaniasis.PLoS neglected tropical diseases · 2020Article
- Evaluation of two multiplex RT-PCR assays for detection and subtype differentiation of Brazilian swine influenza viruses.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- An improved advanced fragment analysis-based classification and risk stratification of pediatric acute lymphoblastic leukemia.Cancer cell international · 2019Article
- Article
- A novel quantitative PCR mediated by high-fidelity DNA polymerase.Scientific reports · 2017Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInfluenza A virus is a leading causative pathogen of human acute respiratory infection. Recently, the co-circulation of pandemic (H1N1) 2009 and seasonal H3N2 viruses was reported, and sporadic cases with reassortant avian H7N9 virus are continually reported in China. We aimed to establish a multiplex one-step real-time reverse transcription-polymerase chain reaction (rRT-PCR) assay to simultaneously detect and discriminate FluA subtypes, including human seasonal H3N2 virus, pandemic (H1N1) 2009 virus and reassortant avian H7N9 virus, in one reaction tube.
methodsClinical samples, including throat swabs and sputum, were collected from the patients with influenza-like illness (ILIs). Total viral RNA from each sample or viral culture was extracted, and the specific detection of FluA virus and its subtypes was performed using a multiplex rRT-PCR assay.
resultsThe limitation of detection (LOD) of the multiplex assay was 5.4 × 10
conclusionsThese findings indicate that the multiplex assay can simultaneously detect and discriminate FluA subtypes with reliable sensitivity and specificity, which is required for the early clinical diagnosis and viral surveillance of patients with FluA infection.
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