Evidence map›Paper›PMID 27994636›Full record

ArticleCytoJournal2016

The effect of the small amount of formaldehyde in the SurePath liquid when establishing protocols for immunocytochemistry.

Ellen Cathrine Bjønness-Jacobsen, Anne Kristine Kallevåg Eriksen, Vidar Nyløkken Hagen, Kirsten Margrethe Østbye, Anna Wittersø, Mette Kristin Pedersen, Torill Sauer

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In one paragraph

Article in CytoJournal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ellen Cathrine Bjønness-JacobsenAddress: Section Biomedical Education, Høgskolen i Oslo og Akershus, Oslo, Norway.
Anne Kristine Kallevåg EriksenAddress: Section Biomedical Education, Høgskolen i Oslo og Akershus, Oslo, Norway.
Vidar Nyløkken HagenAddress: Section Biomedical Education, Høgskolen i Oslo og Akershus, Oslo, Norway.
Kirsten Margrethe ØstbyeAddress: Section Biomedical Education, Høgskolen i Oslo og Akershus, Oslo, Norway.
Anna WittersøDepartment of Pathology, Akershus University Hospital, Lørenskog, Norway.
Mette Kristin PedersenDepartment of Pathology, Akershus University Hospital, Lørenskog, Norway.
Torill SauerDepartment of Pathology, Akershus University Hospital, Lørenskog, Norway; Campus Ahus Faculty of Medicine, Institute of Clinical Medicine, University of Oslo, Oslo, Norway.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSurePath MATERIALS AND

methodsRest material from one malignant and three benign effusions were fixed in SurePath for 1-12 days. Cytospins were incubated with cytokeratin 7 antibody (AB) to evaluate the staining intensity of carcinoma cells and benign, reactive mesothelial cells. Protocols varied as to pretreatment and AB incubation time.

resultsReduced immunostaining intensity was seen within 5 days of storage in the SurePath liquid. It was restored when the pretreatment time was prolonged.

conclusionsThe small amount of formaldehyde in the SurePath liquid seems to affect the immunoreactivity. Local immunocytochemistry protocols in the cytology laboratories should consider this when optimizing their procedures. Postfixation with formalin should be omitted.

Indexed as

Formaldehyde effectimmunocytochemistrySurePath

Identifiers

PMID27994636
PMCPMC5137340

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.