ArticleDevelopment (Cambridge, England)2017
Genetic mosaics and time-lapse imaging identify functions of histone H3.3 residues in mouse oocytes and embryos.
Article in Development (Cambridge, England), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 9 citations in OpenAlex.
- Dynamic nucleosome organization after fertilization reveals regulatory factors for mouse zygotic genome activation.Cell research · 2022Article
- Asymmetrical deposition and modification of histone H3 variants are essential for zygote development.Life science alliance · 2021Article
- Dynamic pattern of histone H3 core acetylation in human early embryos.Cell cycle (Georgetown, Tex.) · 2020Article
- Histone H3 methylation orchestrates transcriptional program in mouse spermatogenic cell line.The Journal of reproduction and development · 2020Article
- Enigma of Retrotransposon Biology in Mammalian Early Embryos and Embryonic Stem Cells.Stem cells international · 2018Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
During development from oocyte to embryo, genetic programs in mouse germ cells are reshaped by chromatin remodeling to orchestrate the onset of development. Epigenetic modifications of specific amino acid residues of core histones and their isoforms can dramatically alter activation and suppression of gene expression. H3.3 is a histone H3 variant that plays essential roles in mouse oocytes and early embryos, but the functional role of individual amino acid residues has been unclear because of technical hurdles. Here, we describe two strategies that successfully investigated the functions of three individual H3.3 residues in oogenesis, cleavage-stage embryogenesis and early development. We first generated genetic mosaic ovaries and blastocysts with stochastic expression of wild-type or mutant H3.3 alleles and showed dominant negative effects of H3.3R26 and H3.3K27 in modulating oogenesis and partitioning cells to the inner cell mass of the early embryo. Time-lapse imaging assays also revealed the essential roles of H3.3K56 in efficient H2B incorporation and paternal pronuclei formation. Application of these strategies can be extended to investigate roles of additional H3.3 residues and has implications for use in other developmental systems.
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Registered trials
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