ArticleMethods (San Diego, Calif.)2017
RNA interactome capture in yeast.
Article in Methods (San Diego, Calif.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Cross-species R-DeeP profiling reveals a conserved core of RNA-dependent proteins in yeast.Nucleic acids research · 2026Article
- RNA-Binding Proteome-Wide Analysis Reveals Rice RNA-Binding Proteins Enriched After Sobemovirus Rice Yellow Mottle Virus Infection.Plant direct · 2025Article
- Article
- Surveying the global landscape of post-transcriptional regulators.Nature structural & molecular biology · 2023Article
- The RNA-bound proteome of MRSA reveals post-transcriptional roles for helix-turn-helix DNA-binding and Rossmann-fold proteins.Nature communications · 2022Article
- easyCLIP analysis of RNA-protein interactions incorporating absolute quantification.Nature communications · 2021Article
- Shaping the Innate Immune Response Through Post-Transcriptional Regulation of Gene Expression Mediated by RNA-Binding Proteins.Frontiers in immunology · 2021Review
- Single and Combined Methods to Specifically or Bulk-Purify RNA-Protein Complexes.Biomolecules · 2020Review
- Discovering the RNA-Binding Proteome of Plant Leaves with an Improved RNA Interactome Capture Method.Biomolecules · 2020Article
- Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.Nature communications · 2019Article
- Methods to study RNA-protein interactions.Nature methods · 2019Review
- Probing the RNA-Binding Proteome from Yeast to Man: Major Advances and Challenges.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Kinetic CRAC uncovers a role for Nab3 in determining gene expression profiles during stress.Nature communications · 2017Article
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA-binding proteins (RBPs) are key players in post-transcriptional regulation of gene expression in eukaryotic cells. To be able to unbiasedly identify RBPs in Saccharomyces cerevisiae, we developed a yeast RNA interactome capture protocol which employs RNA labeling, covalent UV crosslinking of RNA and proteins at 365nm wavelength (photoactivatable-ribonucleoside-enhanced crosslinking, PAR-CL) and finally purification of the protein-bound mRNA. The method can be easily implemented in common workflows and takes about 3days to complete. Next to a comprehensive explanation of the method, we focus on our findings about the choice of crosslinking in yeast and discuss the rationale of individual steps in the protocol.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.