ArticlePloS one2016
Selection and Validation of Reference Genes for Gene Expression Analysis in Vigna angularis Using Quantitative Real-Time RT-PCR.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Identification of best housekeeping gene for normalization in qRT-PCR data under different development stages and stress conditions in Vigna mungo.Scientific reports · 2025Article
- Putative type III effector SkP48 of Bradyrhizobium sp. DOA9 encoding a SUMO protease blocks nodulation in Vigna radiata.Scientific reports · 2025Article
- NopP2 effector of Bradyrhizobium elkanii USDA61 is a determinant of nodulation in Vigna radiata cultivars.Scientific reports · 2024Article
- Article
- Differential Participation of Plant Ribosomal Proteins from the Small Ribosomal Subunit in Protein Translation under Stress.Biomolecules · 2023Article
- Cloning and functional study ofBreeding science · 2023Article
- A natriuretic peptide molecule from Vigna angularis, VaEG45, confers rust resistance by inhibiting fungal development.Plant cell reports · 2023Article
- From plant survival to thriving: exploring the miracle of brassinosteroids for boosting abiotic stress resilience in horticultural crops.Frontiers in plant science · 2023Review
- Histological and molecular responses of Vigna angularis to Uromyces vignae infection.BMC plant biology · 2022Article
- Selection of suitable candidate genes for mRNA expression normalization in bulbil development of Pinellia ternata.Scientific reports · 2022Article
- Article
- Reference gene selection for quantitative RT-PCR in Miscanthus sacchariflorus under abiotic stress conditions.Molecular biology reports · 2022Article
- Selection and Validation of Reference Genes for Quantitative Real-Time PCR Analysis of Development and Tissue-Dependent Flower Color Formation inInternational journal of molecular sciences · 2022Article
- Unraveling Origin, History, Genetics, and Strategies for Accelerated Domestication and Diversification of Food Legumes.Frontiers in genetics · 2022Review
- Identification and Validation of Reference Genes inFrontiers in microbiology · 2021Article
- Pulse Crop Genetics for a Sustainable Future: Where We Are Now and Where We Should Be Heading.Frontiers in plant science · 2020Review
- Identification and validation of reference genes for real-time quantitative RT-PCR analysis in jute.BMC molecular biology · 2019Article
- Validation of reference genes for qRT-PCR data normalisation in lentil (Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Selection and validation of reference genes desirable for gene expression analysis by qRT-PCR in MeJA-treated ginseng hairy roots.PloS one · 2019Article
- Identification and evaluation of PCR reference genes for host and pathogen in sugarcane-Sporisorium scitamineum interaction system.BMC genomics · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adzuki bean (Vigna angularis) is one of the most important legume crops in Asian countries like China, Japan and Korea due to its nutritious protein and starch contents. In spite of its economic importance, gene expression analysis system for gene function verification of adzuki bean is still absent. Therefore, reference genes for gene expression analysis based on the quantitative real time PCR (qRT-PCR) were screened in current study. A total of nine general housekeeping genes, including ACT, Fbox, ZMPP, GAPDH, EF, PP2A, UBC, UBN and PTB were evaluated for their expression stability by qRT-PCR in four adzuki bean cultivars, three different tissues, four abiotic stress and one biotic stress. The best group of candidates as reference genes were as follows: PTB and ACT for different cultivars; EF and UBN for different tissues; ACT and ZMPP for biotic stress and waterlogging stress; Fbox and UBC for salinity-alkalinity stress; Fbox and PTB for drought stress. Our results will provide a more accurate and reliable normalization of qRT-PCR data in adzuki bean.
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