ArticlePloS one2016
Exposure of Bacterial Biofilms to Electrical Current Leads to Cell Death Mediated in Part by Reactive Oxygen Species.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 63 citations in OpenAlex.
- Adjunctive intraoperative pulsed direct current with cefazolin: a rabbit proof-of-concept model for prevention of prosthetic joint infection.Frontiers in cellular and infection microbiology · 2026Article
- Electrolytic cleaning as a biofilm control strategy on medical implants: mechanisms, evidence and translational perspectives.Frontiers in microbiology · 2026Review
- Article
- Optimization of the Extraction Process and Biological Activities of Triterpenoids ofMolecules (Basel, Switzerland) · 2024Article
- Friends' Perspective: Young Adults' Reaction to Disclosure of Chronic Illness.Journal of clinical psychology in medical settings · 2024Article
- Electrochemical biosensors on microfluidic chips as promising tools to study microbial biofilms: a review.Frontiers in cellular and infection microbiology · 2024Review
- The effect of titanium surface treatment by application of constant potential or current on the viability of pre-osteoblast cells: anFrontiers in bioengineering and biotechnology · 2024Article
- Smart dental materials for antimicrobial applications.Bioactive materials · 2023Article
- Mode of killing determines the necrotrophic response of oral bacteria.Journal of oral microbiology · 2023Article
- Physical Approaches to Prevent and Treat Bacterial Biofilm.Antibiotics (Basel, Switzerland) · 2022Review
- Comparisons of the killing effect of direct current partially mediated by reactive oxygen species onJournal of dental sciences · 2022Article
- Direct current exerts electricidal and bioelectric effects on Porphyromonas gingivalis biofilms partially via promoting oxidative stress and antibiotic transport.Journal of microbiology (Seoul, Korea) · 2022Article
- Microbial biofilm: formation, architecture, antibiotic resistance, and control strategies.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Review
- New Trends in Photodynamic Inactivation (PDI) Combating Biofilms in the Food Industry-A Review.Foods (Basel, Switzerland) · 2021Review
- 4-4-(Anilinomethyl)-3-[4-(trifluoromethyl)phenyl]-1H-pyrazol-1-ylbenzoic acid derivatives as potent anti-gram-positive bacterial agents.European journal of medicinal chemistry · 2021Article
- Simultaneous Delivery of Multiple Antimicrobial Agents by Biphasic Scaffolds for Effective Treatment of Wound Biofilms.Advanced healthcare materials · 2021Article
- Review
- Microampere Electric Current Causes Bacterial Membrane Damage and Two-Way Leakage in a Short Period of Time.Applied and environmental microbiology · 2020Article
- Review
- Electroceutical Treatment of Pseudomonas aeruginosa Biofilms.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Bacterial biofilms may form on indwelling medical devices such as prosthetic joints, heart valves and catheters, causing challenging-to-treat infections. We have previously described the 'electricidal effect', in which bacterial biofilms are decreased following exposure to direct electrical current. Herein, we sought to determine if the decreased bacterial quantities are due to detachment of biofilms or cell death and to investigate the role that reactive oxygen species (ROS) play in the observed effect. Using confocal and electron microscopy and flow cytometry, we found that direct current (DC) leads to cell death and changes in the architecture of biofilms formed by Gram-positive and Gram-negative bacteria. Reactive oxygen species (ROS) appear to play a role in DC-associated cell death, as there was an increase in ROS-production by Staphylococcus aureus and Staphylococcus epidermidis biofilms following exposure to DC. An increase in the production of ROS response enzymes catalase and superoxide dismutase (SOD) was observed for S. aureus, S. epidermidis and Pseudomonas aeruginosa biofilms following exposure to DC. Additionally, biofilms were protected from cell death when supplemented with antioxidants and oxidant scavengers, including catalase, mannitol and Tempol. Knocking out SOD (sodAB) in P. aeruginosa led to an enhanced DC effect. Microarray analysis of P. aeruginosa PAO1 showed transcriptional changes in genes related to the stress response and cell death. In conclusion, the electricidal effect results in death of bacteria in biofilms, mediated, at least in part, by production of ROS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.