ArticleBritish journal of haematology2017
Molecular typing for blood group antigens within 40 min by direct polymerase chain reaction from plasma or serum.
Article in British journal of haematology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Influence of ABO blood group on susceptibility to different pathological types of lung cancer: a retrospective study.World journal of surgical oncology · 2022Article
- ABO blood classification and the risk of lung cancer: A meta-analysis and trial sequential analysis.Oncology letters · 2022Article
- Genotyping of Single Nucleotide Polymorphisms Using Allele-Specific qPCR Producing Amplicons of Small Sizes Directly from Crude Serum Isolated from Capillary Blood by a Hand-Powered Paper Centrifuge.Diagnostics (Basel, Switzerland) · 2019Article
- Molecular immunohaematology round table discussions at the AABB Annual Meeting, Orlando 2016.Blood transfusion = Trasfusione del sangue · 2018Article
- Serological weak D phenotypes: a review and guidance for interpreting the RhD blood type using the RHD genotype.British journal of haematology · 2017Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Determining blood group antigens by serological methods may be unreliable in certain situations, such as in patients after chronic or massive transfusion. Red cell genotyping offers a complementary approach, but current methods may take much longer than conventional serological typing, limiting their utility in urgent situations. To narrow this gap, we devised a rapid method using direct polymerase chain reaction (PCR) amplification while avoiding the DNA extraction step. DNA was amplified by PCR directly from plasma or serum of blood donors followed by a melting curve analysis in a capillary rapid-cycle PCR assay. We evaluated the single nucleotide polymorphisms underlying the clinically relevant Fy
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Registered trials
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