ArticleScientific reports2016
Genes encoding norcoclaurine synthase occur as tandem fusions in the Papaveraceae.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 43 citations in OpenAlex.
- Amaryllidaceae Alkaloids and Isoquinoline Alkaloids: A Perspective on Historical Approaches to Pathway Elucidation.Plants (Basel, Switzerland) · 2026Review
- Neuroprotective mechanisms of nutraceutical berberine: Preclinical evidence and future perspectives.Advances in neurotoxicology · 2026Article
- Genome-Wide Identification and Functional Analysis of the Norcoclaurine Synthase Gene Family inInternational journal of molecular sciences · 2025Article
- Molecular and expression analyses indicate the role of fusion transcripts in mediating abiotic stress responses in chickpea.Frontiers in plant science · 2025Article
- Herbgenomics meets Papaveraceae: a promising -omics perspective on medicinal plant research.Briefings in functional genomics · 2024Review
- Genome-wide identification of rubber tree pathogenesis-related 10 (PR-10) proteins with biological relevance to plant defense.Scientific reports · 2024Article
- Elucidation of the (R)-enantiospecific benzylisoquinoline alkaloid biosynthetic pathways in sacred lotus (Nelumbo nucifera).Scientific reports · 2023Article
- Plant biosynthetic gene clusters in the context of metabolic evolution.Natural product reports · 2022Review
- Machine learning discovery of missing links that mediate alternative branches to plant alkaloids.Nature communications · 2022Article
- Biosynthesis and synthetic biology of psychoactive natural products.Chemical Society reviews · 2021Review
- Isolation and characterization of twoThe Journal of biological chemistry · 2020Article
- Article
- Engineering Plant Secondary Metabolism in Microbial Systems.Plant physiology · 2019Article
- Cloning and characterization of norbelladine synthase catalyzing the first committed reaction in Amaryllidaceae alkaloid biosynthesis.BMC plant biology · 2018Article
- Benzylisoquinoline Alkaloids Biosynthesis in Sacred Lotus.Molecules (Basel, Switzerland) · 2018Review
- Complete biosynthesis of noscapine and halogenated alkaloids in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Development of a Method to Extract Opium Poppy (Papaver somniferum L.) DNA from Heroin.Scientific reports · 2018Article
- ThePlant physiology · 2018Article
- Article
- Enzyme catalysed Pictet-Spengler formation of chiral 1,1'-disubstituted- and spiro-tetrahydroisoquinolines.Nature communications · 2017Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Norcoclaurine synthase (NCS) catalyzes the enantioselective Pictet-Spengler condensation of dopamine and 4-hydroxyphenylacetaldehyde as the first step in benzylisoquinoline alkaloid (BIA) biosynthesis. NCS orthologs in available transcriptome databases were screened for variants that might improve the low yield of BIAs in engineered microorganisms. Databases for 21 BIA-producing species from four plant families yielded 33 assembled contigs with homology to characterized NCS genes. Predicted translation products generated from nine contigs consisted of two to five sequential repeats, each containing most of the sequence found in single-domain enzymes. Assembled contigs containing tandem domain repeats were detected only in members of the Papaveraceae family, including opium poppy (Papaver somniferum). Fourteen cDNAs were generated from 10 species, five of which encoded NCS orthologs with repeated domains. Functional analysis of corresponding recombinant proteins yielded six active NCS enzymes, including four containing either two, three or four repeated catalytic domains. Truncation of the first 25 N-terminal amino acids from the remaining polypeptides revealed two additional enzymes. Multiple catalytic domains correlated with a proportional increase in catalytic efficiency. Expression of NCS genes in Saccharomyces cereviseae also produced active enzymes. The metabolic conversion capacity of engineered yeast positively correlated with the number of repeated domains.
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