ArticleScientific reports2016
Quantitative assessment of the regenerative and mineralogenic performances of the zebrafish caudal fin.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- Article
- Lack of Nuclear Localization of the Creb3l1 Transcription Factor Causes Defects in Caudal Fin Bifurcation in Zebrafish Danio rerio.Cells, tissues, organs · 2025Article
- Immunomodulatory inhibition of osteoclastogenesis by a marine microalgal ethanol fraction targeting T-cells, antigen presentation, and macrophage fate.Frontiers in immunology · 2025Article
- Disruption of the creb3l1 gene causes defects in caudal fin regeneration and patterning in zebrafish Danio rerio.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Article
- The osteogenic and mineralogenic potential of the microalgae Skeletonema costatum and Tetraselmis striata CTP4 in fish models.Cellular and molecular life sciences : CMLS · 2023Article
- Zebrafish Tric-b is required for skeletal development and bone cells differentiation.Frontiers in endocrinology · 2023Article
- Fin ray branching is defined by TRAPProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Screening of Mineralogenic and Osteogenic Compounds in Zebrafish-Tools to Improve Assay Throughput and Data Accuracy.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Zebrafish caudal fin as a model to investigate the role of probiotics in bone regeneration.Scientific reports · 2022Article
- Influence of Prednisolone and Alendronate on theJBMR plus · 2021Article
- Vitamin K in Vertebrates' Reproduction: Further Puzzling Pieces of Evidence from Teleost Fish Species.Biomolecules · 2020Review
- New Insights on Vitamin K Metabolism in Senegalese sole (International journal of molecular sciences · 2020Article
- Zebrafish: A Resourceful Vertebrate Model to Investigate Skeletal Disorders.Frontiers in endocrinology · 2020Review
- New Insights into the Control of Cell Fate Choices and Differentiation by Retinoic Acid in Cranial, Axial and Caudal Structures.Biomolecules · 2019Review
- Zebrafish Models of Human Skeletal Disorders: Embryo and Adult Swimming Together.BioMed research international · 2019Review
- Bone Research Society, Annual Meeting 2017 Proceedings. 25-27 June 2017, Bristol, UK.Journal of musculoskeletal & neuronal interactions · 2018Article
- Micro-CT - a digital 3D microstructural voyage into scaffolds: a systematic review of the reported methods and results.Biomaterials research · 2018Review
- Screening for osteogenic activity in extracts from Irish marine organisms: The potential of Ceramium pallidum.PloS one · 2018Article
- Micro-computed tomography characterization of tissue engineering scaffolds: effects of pixel size and rotation step.Journal of materials science. Materials in medicine · 2017Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of zebrafish to fully regenerate its caudal fin has been explored to better understand the mechanisms underlying de novo bone formation and to develop screening methods towards the discovery of compounds with therapeutic potential. Quantifying caudal fin regeneration largely depends on successfully measuring new tissue formation through methods that require optimization and standardization. Here, we present an improved methodology to characterize and analyse overall caudal fin and bone regeneration in adult zebrafish. First, regenerated and mineralized areas are evaluated through broad, rapid and specific chronological and morphometric analysis in alizarin red stained fins. Then, following a more refined strategy, the intensity of the staining within a 2D longitudinal plane is determined through pixel intensity analysis, as an indicator of density or thickness/volume. The applicability of this methodology on live specimens, to reduce animal experimentation and provide a tool for in vivo tracking of the regenerative process, was successfully demonstrated. Finally, the methodology was validated on retinoic acid- and warfarin-treated specimens, and further confirmed by micro-computed tomography. Because it is easily implementable, accurate and does not require sophisticated equipment, the present methodology will certainly provide valuable technical standardization for research in tissue engineering, regenerative medicine and skeletal biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.