Evidence map›Paper›PMID 27990449›Full record

ArticleMolecular therapy. Methods & clinical development2016

Elimination of proliferating cells from CNS grafts using a Ki67 promoter-driven thymidine kinase.

Vannary Tieng, Ophelie Cherpin, Eveline Gutzwiller, Alexander C Zambon, Christophe Delgado, Patrick Salmon, Michel Dubois-Dauphin, Karl-Heinz Krause

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular therapy. Methods & clinical development, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.1field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 21 citations in OpenAlex.

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  14. The Nexus of Stem Cell-Derived Beta-Cells and Genome Engineering.The review of diabetic studies : RDS · 2017
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Vannary TiengDepartment of pathology and immunology, Geneva medical school, University of Geneva, Geneva, Switzerland; Department of genetic medicine and laboratory, Geneva Hospitals, Geneva, Switzerland.
Ophelie CherpinDepartment of pathology and immunology, Geneva medical school, University of Geneva , Geneva, Switzerland.
Eveline GutzwillerDepartment of pathology and immunology, Geneva medical school, University of Geneva , Geneva, Switzerland.
Alexander C ZambonDepartment of Biopharmaceutical Science, Keck Graduate Institute , Claremont, California, USA.
Christophe DelgadoDepartment of pathology and immunology, Geneva medical school, University of Geneva , Geneva, Switzerland.
Patrick SalmonDepartment of basic neurosciences, Geneva medical school, University of Geneva , Geneva, Switzerland.
Michel Dubois-DauphinDepartment of pathology and immunology, Geneva medical school, University of Geneva , Geneva, Switzerland.
Karl-Heinz KrauseDepartment of pathology and immunology, Geneva medical school, University of Geneva, Geneva, Switzerland; Department of genetic medicine and laboratory, Geneva Hospitals, Geneva, Switzerland.
University of Geneva · CHUniversity Hospital of Geneva · CHKeck Graduate Institute · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pluripotent stem cell (PSC)-based cell therapy is an attractive concept for neurodegenerative diseases, but can lead to tumor formation. This is particularly relevant as proliferating neural precursors rather than postmitotic mature neurons need to be transplanted. Thus, safety mechanisms to eliminate proliferating cells are needed. Here, we propose a suicide gene approach, based on cell cycle-dependent promoter Ki67-driven expression of herpes simplex virus thymidine kinase (HSV-TK). We generated a PSC line expressing this construct and induced neural differentiation

Identifiers

PMID27990449
PMCPMC5129875
OpenAlexW2558463855

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.