Evidence map›Paper›PMID 27987408›Full record

ArticleBiosensors & bioelectronics2017

Label-free electrical sensing of bacteria in eye wash samples: A step towards point-of-care detection of pathogens in patients with infectious keratitis.

Hardik J Pandya, Manoj Kumar Kanakasabapathy, Saloni Verma, Manjyot Kaur Chug, Adnan Memic, Mihaela Gadjeva, Hadi Shafiee

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Microfluidics for Environmental Applications.Advances in biochemical engineering/biotechnology · 2022
    Review
  3. Article
  4. Article
  5. Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Hardik J PandyaDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital - Harvard Medical School, Boston, MA 02115, USA.
Manoj Kumar KanakasabapathyDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital - Harvard Medical School, Boston, MA 02115, USA.
Saloni VermaDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital - Harvard Medical School, Boston, MA 02115, USA.
Manjyot Kaur ChugDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital - Harvard Medical School, Boston, MA 02115, USA.
Adnan MemicCenter for Nanotechnology, King AbdulAziz University, Jeddah 21589, Saudi Arabia.
Mihaela GadjevaDivision of Infectious Disease, Department of Medicine, Brigham and Women's Hospital - Harvard Medical School, Boston, MA 02115, USA; Department of Medicine, Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, USA.
Hadi ShafieeDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital - Harvard Medical School, Boston, MA 02115, USA; Department of Medicine, Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, USA. Electronic address: HSHAFIEE@BWH.HARVARD.EDU.
Brigham and Women's Hospital · USHarvard University · USKing Abdulaziz University · SA

Funding

Novel treatment strategies to regulate inflammation during P.aeruginosa keratitisR01EY022054 · NEI · BRIGHAM AND WOMEN'S HOSPITAL · PI GADJEVA, MIHAELA GUEORGUIEVA · 2012 to 2020
$4.0M
Sensitive and quantitative point-of-care viral load testing on paperR01AI118502 · NIAID · BRIGHAM AND WOMEN'S HOSPITAL · PI SHAFIEE, HADI · 2016 to 2020
$2.2M
NEI NIH HHS R01 EY022054NIAID NIH HHS R01 AI118502
6 · The paper itself

Abstract

The diagnosis of keratitis is based on visual exam, tissue cytology, and standard microbial culturing to determine the type of the infectious pathogen. To prescribe appropriate therapy, it is important to distinguish between bacterial, fungal, and viral keratitis, as the treatments are quite different. Diagnosis of the causative organism has a substantial prognostic importance. Further, timely knowledge of the nature of the pathogen is also critical to adapt therapy in patients unresponsive to empiric treatment options, which occurs in 10% of all cases. Currently, the identification of the nature of the pathogen that causes keratitis is achieved via microbial culture screening, which is laboratory-based, expensive, and time-consuming. The most frequent pathogens that cause the corneal ulcers are P. aeruginosa and S. aureus. Here, we report a microchip for rapid (<1h) detection of P. aeruginosa (6294), S. aureus(LAC), through on-chip electrical sensing of bacterial lysate. We evaluated the microchip with spiked samples of PBS with bacteria concentration between 10

Indexed as

Point-of-Care SystemsAnimalsBiosensing TechniquesElectric ImpedanceEquipment DesignHumansKeratitisLab-On-A-Chip DevicesLimit of DetectionMiceMice, Inbred C57BLPseudomonas aeruginosaPseudomonas InfectionsStaphylococcal InfectionsStaphylococcus aureusTearsBacterial lysateElectrical sensingKeratitisMicrosensorPoint-of-care detection

Identifiers

PMID27987408
PMCPMC5323318
OpenAlexW2565048730

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.